US 11,899,011 B2
Selective optical detection of organic analytes in liquids
Sergei Babitshenko, Tallinn (EE); Jaak Järv, Tallinn (EE); Aleksei Kuznetsov, Tallinn (EE); and Anton Mastitski, Tallinn (EE)
Assigned to QanikDX OU, Tallinn (EE)
Filed by QanikDX OÜ, Tallinn (EE)
Filed on Apr. 27, 2023, as Appl. No. 18/308,219.
Application 18/308,219 is a continuation of application No. 17/251,536, granted, now 11,650,205, previously published as PCT/EP2020/051268, filed on Jan. 20, 2020.
Claims priority of application No. U201900011 (EE), filed on Feb. 11, 2019.
Prior Publication US 2023/0258631 A1, Aug. 17, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. G01N 33/543 (2006.01); G01N 33/58 (2006.01); G01N 21/64 (2006.01)
CPC G01N 33/54373 (2013.01) [G01N 21/6428 (2013.01); G01N 33/582 (2013.01); G01N 33/588 (2013.01); G01N 2021/6441 (2013.01)] 20 Claims
OG exemplary drawing
 
1. An assay substrate comprising:
a first component comprising a sensor molecule labeled with a quantum dot, the quantum dot immobilized to an assay substrate surface with a first linker, the first linker being a bi-polar linker comprising a first binding group for specific binding of the quantum dot and a second binding group for specific binding of the assay substrate surface, the sensor molecule having a specific binding site for an organic analyte, the sensor molecule labeled with the quantum dot in a position that has no effect on the organic analyte binding the specific binding site;
a second component comprising a chemical analogue of the organic analyte, the chemical analogue labeled with a fluorescent dye, the chemical analogue linked to the quantum dot with a second linker having a length exceeding Förster radius, and the chemical analogue reversibly binding the specific binding site of the sensor molecule of the first component;
the quantum dot having a first fluorescence emission spectrum that excites fluorescence of the fluorescent dye and the fluorescent dye having a second fluorescence emission spectrum distinguished from the first fluorescence spectrum of the quantum dot.