US 11,898,961 B2
System and method for detecting a periprosthetic infection
Forrest Miller, Philadelphia, PA (US); Samit Kumar Gupta, Menlo Park, CA (US); David John Kuraguntla, Bel Air, MD (US); and Elsa Swanson, Cherry Hill, NJ (US)
Assigned to Alio, Inc., San Francisco, CA (US)
Filed by ALIO, Inc., Broomfield, CO (US)
Filed on Apr. 2, 2020, as Appl. No. 16/838,253.
Application 16/838,253 is a continuation of application No. PCT/US2018/050675, filed on Sep. 12, 2018.
Claims priority of provisional application 62/558,259, filed on Sep. 13, 2017.
Prior Publication US 2020/0232983 A1, Jul. 23, 2020
Int. Cl. G01N 33/50 (2006.01); G01N 21/51 (2006.01); G01N 21/84 (2006.01); G01N 33/543 (2006.01)
CPC G01N 21/8483 (2013.01) [G01N 21/51 (2013.01); G01N 33/5094 (2013.01); G01N 2333/4737 (2013.01); G01N 2333/914 (2013.01); G01N 2800/26 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A system for detecting an infection in a fluid sample drawn from a patient at the patient's point-of-care, the system comprising:
a white blood cell (WBC) concentrator, the WBC concentrator comprising:
i. a vial receptacle sized to receive a vial containing the fluid sample;
ii. one or more light sources operable to illuminate the fluid sample in the vial; and
iii. a vial optical sensor oriented to detect scattered light intensity, absorbed light intensity, or both by white blood cells in the fluid sample, wherein the vial optical sensor is configured to convert the light intensity to a vial electrical signal;
at least one immunoassay (IA) strip reader comprising:
i. at least one IA strip receptacle sized to receive an IA strip having an analyte reagent and a portion of the fluid sample, wherein the analyte reagent is configured to generate an analyte light intensity from a reaction between the analyte reagent and an analyte in the fluid sample; and
ii. an IA strip optical sensor oriented to detect the analyte light intensity, wherein the IA strip optical sensor is configured to convert the analyte light intensity to an IA analyte electrical signal; and
an optical sensor reader, the optical sensor reader comprising a processor configured to receive the vial electrical signals and the IA analyte electrical signals, to store the vial electrical signals as vial sensor data and the IA analyte electrical signals as IA analyte sensor data, and to communicate the vial sensor data and the IA analyte sensor data over a communications interface, the processor in communication with the communications interface configured to determine a WBC concentration from the vial sensor data and a leukocyte concentration and from the IA analyte sensor data.