US 11,898,196 B2
Method for isolating target nucleic acid using heteroduplex binding proteins
Andrea Marziali, North Vancouver (CA); Milenko Despotovic, Richmond (CA); Matthew Wiggin, Vancouver (CA); and Joel Pel, Vancouver (CA)
Assigned to Quantum-Si Incorporated, Branford, CT (US)
Filed by Quantum-Si Incorporated, Guilford, CT (US)
Filed on Aug. 18, 2021, as Appl. No. 17/405,083.
Application 17/405,083 is a continuation of application No. 15/575,699, granted, now 11,130,986, previously published as PCT/IB2016/000796, filed on May 19, 2016.
Claims priority of provisional application 62/291,222, filed on Feb. 4, 2016.
Claims priority of provisional application 62/164,247, filed on May 20, 2015.
Prior Publication US 2022/0056506 A1, Feb. 24, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6811 (2018.01); C12Q 1/6806 (2018.01); C12Q 1/6869 (2018.01); C12Q 1/686 (2018.01); C12Q 1/6874 (2018.01)
CPC C12Q 1/6811 (2013.01) [C12Q 1/6806 (2013.01); C12Q 1/686 (2013.01); C12Q 1/6869 (2013.01); C12Q 1/6874 (2013.01)] 16 Claims
 
1. A method for separating target heteroduplex nucleic acid from background nucleic acid, comprising;
nicking the target heteroduplex nucleic acid;
loading a mixture of nicked target heteroduplex nucleic acid and background homoduplex nucleic acid on a separation medium, wherein the target heteroduplex nucleic acid and the background homoduplex nucleic acid differ by at least one base;
contacting the mixture of target heteroduplex nucleic acid and background homoduplex nucleic acid with a heteroduplex-binding protein to create a heteroduplex-binding protein-nucleic acid mixture;
applying a time-varying driving field to the separation medium; and
applying a time-varying mobility varying field to the separation medium,
thereby causing the target heteroduplex nucleic acid to be separated from the background homoduplex nucleic acid.