US 11,898,194 B2
Method for detecting target molecule
Takumi Hirase, Taito-ku (JP); Yoichi Makino, Taito-ku (JP); and Yosuke Horiuchi, Taito-ku (JP)
Assigned to TOPPAN PRINTING CO., LTD., Taito-ku (JP)
Filed by TOPPAN PRINTING CO., LTD., Taito-ku (JP)
Filed on May 12, 2020, as Appl. No. 16/872,855.
Application 16/872,855 is a continuation of application No. PCT/JP2018/042362, filed on Nov. 15, 2018.
Claims priority of application No. 2017-222128 (JP), filed on Nov. 17, 2017.
Prior Publication US 2020/0270674 A1, Aug. 27, 2020
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6806 (2018.01); B01L 3/00 (2006.01); C12Q 1/686 (2018.01); G01N 33/536 (2006.01)
CPC C12Q 1/6806 (2013.01) [B01L 3/5027 (2013.01); C12Q 1/686 (2013.01); G01N 33/536 (2013.01); B01L 2200/0689 (2013.01); B01L 2300/0829 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method for detecting a target molecule, comprising:
providing a fluid device having a flow path and a plurality of wells arrayed in the fluid device and connected to the flow path;
supplying a liquid dispersion of a structural substance having a target molecule into the fluid device such that the structural substance is distributed into the plurality of wells in the fluid device and has the target molecule in or on the structural substance;
supplying a sealing solution into the fluid device such that a layer of the sealing solution is formed in the flow path in the fluid device and individually seals the plurality of wells containing the liquid dispersion;
extracting the target molecule from the structural substance in the plurality of wells sealed by the layer of the sealing solution in the fluid device such that the liquid dispersion in the plurality of wells includes the structural substance and the target molecule extracted from the structural substance; and
detecting the target molecule extracted from the structural substance in the plurality of wells in the fluid device,
wherein the fluid device includes a substrate and a cover member attached to the substrate such that the plurality of wells is formed in the substrate and that, the flow path is formed between the substrate and the cover member, and the supplying of the sealing solution includes forming the layer of the sealing solution between the substrate and the cover member such that the layer of the sealing solution entirely fills the flow path formed between the substrate and the cover member.