US 11,898,190 B2
Labyrinthulid microorganism capable of producing microbial oil, microbial oil, methods for producing said microorganism and for producing said microbial oil, and uses of said microorganism and said microbial oil
Yuji Okita, Hachioji (JP); Makoto Ito, Fukuoka (JP); Rie Hamaguchi, Fukuoka (JP); Hatsumi Goda, Fukuoka (JP); Seiya Mochinaga, Fukuoka (JP); and Daisuke Honda, Kobe (JP)
Assigned to KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, Fukuoka (JP); KONAN GAKUEN, Kobe (JP); and NIPPON SUISAN KAISHA, LTD., Tokyo (JP)
Filed by KYUSHU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION, Fukuoka (JP); KONAN GAKUEN, Kobe (JP); and NIPPON SUISAN KAISHA, LTD., Tokyo (JP)
Filed on Aug. 13, 2021, as Appl. No. 17/401,894.
Application 17/401,894 is a division of application No. 15/740,969, abandoned, previously published as PCT/JP2016/069825, filed on Jul. 4, 2016.
Claims priority of application No. 2015-134715 (JP), filed on Jul. 3, 2015.
Prior Publication US 2022/0042052 A1, Feb. 10, 2022
Int. Cl. C12P 7/6432 (2022.01); C12N 15/09 (2006.01); C12P 7/64 (2022.01); C12P 1/00 (2006.01); C12N 15/79 (2006.01); C12P 7/6427 (2022.01); C12P 7/6472 (2022.01); C12P 19/34 (2006.01); C12N 1/00 (2006.01); C12N 1/12 (2006.01); C12R 1/00 (2006.01); C12R 1/89 (2006.01); C10G 3/00 (2006.01); C12N 15/52 (2006.01)
CPC C12P 7/6432 (2022.01) [C12N 15/09 (2013.01); C12N 15/79 (2013.01); C12P 1/00 (2013.01); C12P 7/64 (2013.01); C12P 7/6427 (2013.01); C12P 7/6472 (2013.01); C12P 19/34 (2013.01); C10G 3/00 (2013.01); C12N 1/00 (2013.01); C12N 1/125 (2021.05); C12N 15/52 (2013.01); C12R 2001/00 (2021.05); C12R 2001/89 (2021.05)] 4 Claims
 
1. A method for producing a microbial oil, comprising:
genetically modifying a labyrinthulid by disrupting and/or silencing a fatty acid desaturase gene;
culturing the labyrinthulid; and
collecting the microbial oil from the labyrinthulid,
wherein the labyrinthulid before the modification belongs to the genus Parietichytrium or the genus Schizochytrium having an activity of synthesizing DHA via a PUFA-PKS pathway in an amount of not more than 1/100 of a total amount of DHA synthesized in the labyrinthulid or no activity of producing PUFAs via the PUFA-PKS pathway, and having an activity of producing PUFAs via an endogenous elongase-desaturase pathway,
wherein the fatty acid desaturase gene is a Δ4 desaturase gene,
wherein the microbial oil satisfies (a) and (b):
(a) DHA is not greater than 0.50% of the total fatty acid composition; and
(b) a total of DHA and n-6 DPA is not greater than 0.7% of the total fatty acid composition.