	US 11,898,188 B2
	Mutant microorganism having ability to produce 1,3-propanediol, and method for preparing 1,3-PDO by using same
Sang Yup Lee, Daejeon (KR); Jae Sung Cho, Daejeon (KR); Je Woong Kim, Daejeon (KR); Yoo Sung Ko, Daejeon (KR); Cindy Pricilia Surya Prabowo, Daejeon (KR); Taehee Han, Daejeon (KR); Euiduk Kim, Daejeon (KR); Jae Won Choi, Daejeon (KR); Changhee Cho, Daejeon (KR); and Jun Kyu Lee, Daejeon (KR)
Assigned to HANWHA SOLUTIONS CORPORATION, Seoul (KR); and KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, Daejeon (KR)
Appl. No. 16/962,213
Filed by KOREA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, Daejeon (KR); and HANWHA SOLUTIONS CORPORATION, Seoul (KR)
PCT Filed Jan. 15, 2019, PCT No. PCT/KR2019/000571 § 371(c)(1), (2) Date Jul. 14, 2020, PCT Pub. No. WO2019/143089, PCT Pub. Date Jul. 25, 2019.
Claims priority of application No. 10-2018-0005451 (KR), filed on Jan. 16, 2018.
Prior Publication US 2021/0062232 A1, Mar. 4, 2021
Int. Cl. C12P 7/18 (2006.01); C12N 9/04 (2006.01); C12N 9/12 (2006.01); C12N 9/88 (2006.01); C12N 15/77 (2006.01); C12N 1/21 (2006.01)

CPC C12P 7/18 (2013.01) [C12N 9/0006 (2013.01); C12N 9/1205 (2013.01); C12N 9/88 (2013.01); C12N 15/77 (2013.01); C12Y 101/01006 (2013.01); C12Y 101/01202 (2013.01); C12Y 207/0103 (2013.01); C12Y 402/0103 (2013.01)]

2 Claims

1. A method of producing 1,3-propanediol from glycerol, the method comprising steps of:

(a) culturing a mutant microorganism, the mutant microorganism being a mutant of a microorganism Corynebacterium glutamicum spp., MBEL-HCC-C-13PDO1 deposited as KCTC Accession Number 15328BP, transformed with a glycerol facilitator-encoding gene glpF, a glycerol kinase-encoding gene glpK, a glycerol-3-phosphate dehydrogenase-encoding gene glpD, a glycerol dehydratase and glycerol reactivase encoding gene cluster pduCDEGH, and 1,3-PDO oxidoreductase-encoding gene yqhD, wherein the culturing is carried out in a glycerol- and glucose-containing medium, in which a weight ratio of the glucose to glycerol in the medium is 1:2 to 9, thereby producing 1,3-propanediol; and

(b) collecting the produced 1,3-propanediol.