US 11,898,183 B2
Programmed microorganisms to attenuate a disease
Panchapagesa Muthuswamy Murali, Tamil Nadu (IN); Arumbuliyur Sathish Kumar, Tamil Nadu (IN); and Shriram Raghavan, Tamil Nadu (IN)
Filed by Panchapagesa Muthuswamy Murali, Tamil Nadu (IN); Arumbuliyur Sathish Kumar, Tamil Nadu (IN); and Shriram Raghavan, Tamil Nadu (IN)
Filed on Jun. 10, 2021, as Appl. No. 17/344,265.
Claims priority of provisional application 63/058,662, filed on Jul. 30, 2020.
Prior Publication US 2022/0033867 A1, Feb. 3, 2022
Int. Cl. C12P 17/04 (2006.01); A61K 31/7048 (2006.01); A61K 35/74 (2015.01); C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 9/00 (2006.01)
CPC C12P 17/04 (2013.01) [A61K 31/7048 (2013.01); A61K 35/74 (2013.01); C12N 9/0004 (2013.01); C12N 9/0006 (2013.01); C12N 9/0042 (2013.01); C12N 9/0069 (2013.01); C12N 9/0071 (2013.01); C12N 9/0073 (2013.01); C12N 9/1007 (2013.01); C12N 9/1029 (2013.01); C12N 9/88 (2013.01); C12N 9/93 (2013.01); C12Y 101/01331 (2015.07); C12Y 106/02004 (2013.01); C12Y 114/13036 (2013.01); C12Y 201/01104 (2013.01); C12Y 203/01099 (2013.01); C12Y 403/01024 (2013.01); C12Y 602/01012 (2013.01); C07K 2319/00 (2013.01)] 17 Claims
 
1. A recombinant microbe comprising genes encoding phenyl alanine ammonia-lyase (PAL), cinnamate-4-hydroxylate 4-coumaroyl CoA-ligase fusion (C4H4CL), hydroxycinnamoyl-CoA quinate hydroxycinnamoyltransferase p-coumaroyl quinate 3′-hydroxylase fusion (HCTC3H), caffeoyl CoA O-methyltransferase (CCoAOMT), bifunctional pinoresinol-lariciresinol reductase (DIRPLR), secoisolariciresinol dehydrogenase (SDH), O-methyltransferase (OMT), 2-oxoglutarate/Fe(II)-dependent dioxygenase (2-ODD), 2-Deoxy-d-ribose-5-phosphate aldolase, Cytochrome P450 oxidoreductase CYP719, Cytochrome P450 oxidoreductase CYP71 Cytochrome P450 oxidoreductase CYP82D, and UDP glucosyl transferase, and a protein transporter, wherein the recombinant microbe secretes etoposide, or its derivatives.