US 11,898,177 B2
Variant maltohexaose-forming alpha-amylase variants
Luis G Cascao-Pereira, Redwood City, CA (US); David A Estell, San Mateo, CA (US); Marc Anton Bernhard Kolkman, Oegstgeest (NL); and Harm Mulder, Voorhout (NL)
Assigned to DANISCO US INC., Palo Alto, CA (US)
Filed by DANISCO US INC., Palo Alto, CA (US)
Filed on Jul. 12, 2021, as Appl. No. 17/373,307.
Application 17/373,307 is a continuation of application No. 16/572,804, filed on Sep. 17, 2019, abandoned.
Application 16/572,804 is a continuation of application No. 15/160,624, filed on May 20, 2016, granted, now 10,457,924.
Application 15/160,624 is a continuation of application No. 14/354,425, abandoned, previously published as PCT/US2012/062209, filed on Oct. 26, 2012.
Claims priority of provisional application 61/668,359, filed on Jul. 5, 2012.
Claims priority of provisional application 61/552,910, filed on Oct. 28, 2011.
Prior Publication US 2022/0002696 A1, Jan. 6, 2022
Int. Cl. C12N 9/28 (2006.01); A21D 8/04 (2006.01); C11D 3/386 (2006.01); D06L 1/14 (2006.01); D06L 4/40 (2017.01)
CPC C12N 9/2417 (2013.01) [A21D 8/042 (2013.01); C11D 3/386 (2013.01); C12Y 302/01001 (2013.01); D06L 1/14 (2013.01); D06L 4/40 (2017.01)] 8 Claims
 
1. A variant α-amylase polypeptide derived from a parental α-amylase polypeptide, comprising at least two combinable mutations at productive amino acid positions; wherein the mutations are at position H1 and E391, wherein the variant has at least 90% amino acid sequence identity with SEQ ID NO: 3.