US 11,898,154 B2
Enhanced transgene expression and processing
Valerie Le Fourn, Plan-les-Ouates (CH); Nicolas Mermod, Plan-les-Ouates (CH); Alexandre Regamey, Plan-les-Ouates (CH); Montse Buceta, Plan-les-Ouates (CH); Deborah Ley, Plan-les-Ouates (CH); Niamh Harraghy, Plan-les-Ouates (CH); Kaja Kostyrko, Plan-les-Ouates (CH); Pierre-Alain Giro, Plan-les-Ouates (CH); and David Calabrese, Plan-les-Ouates (CH)
Assigned to SELEXIS S.A., Plan-les-Ouates (CH)
Appl. No. 14/761,460
Filed by Selexis S.A., Plan-les-Ouates (CH)
PCT Filed Feb. 1, 2014, PCT No. PCT/IB2014/000100
§ 371(c)(1), (2) Date Jul. 16, 2015,
PCT Pub. No. WO2014/118619, PCT Pub. Date Aug. 7, 2014.
Claims priority of provisional application 61/759,802, filed on Feb. 1, 2013.
Claims priority of provisional application 61/800,244, filed on Mar. 15, 2013.
Claims priority of provisional application 61/806,634, filed on Mar. 29, 2013.
Prior Publication US 2015/0361451 A1, Dec. 17, 2015
Int. Cl. C12N 15/85 (2006.01); C07K 16/00 (2006.01); C07K 16/24 (2006.01); C07K 16/32 (2006.01); C12N 15/90 (2006.01)
CPC C12N 15/85 (2013.01) [C07K 16/00 (2013.01); C07K 16/241 (2013.01); C07K 16/32 (2013.01); C12N 15/90 (2013.01); C07K 2317/10 (2013.01); C07K 2317/24 (2013.01); C12N 2800/107 (2013.01); C12N 2800/90 (2013.01); C12N 2830/46 (2013.01)] 38 Claims
 
1. A recombinant nucleic acid molecule that is part of a transposon vector comprising:
a) a 5′ transposon-specific inverted terminal repeat (ITR) and a 3′ ITR, and
b) located between the 5′ ITR and the 3′ ITR:
(i) at least one nucleic acid sequence encoding a transgene expression processing (TEP) protein under the control of a promoter,
wherein the TEP is selected from the group consisting of human Signal Recognition Particle 14 (hSRP14), human Protein Transport Protein 61α1 (hSec61α1), human Protein Transport Protein 61β (hSec61β), human Protein Transport Protein 61γ (hSec61γ), human Signal Recognition Particle 54 (hSRP54), human Signal Recognition Particle 9 (hSRP9), human Signal Recognition Particle Receptor α (hSRPRα), human Signal Recognition Particle β (hSRPβ), human Calnexin (hCANX), and combinations thereof, and
(ii) a matrix attachment region (MAR) element,
wherein the transposon vector increases an expression of a transgene encoding a product of interest in a mammalian cell by at least 10% relative to a plasmid vector comprising the TEP protein.