	US 11,898,151 B2
	Obtaining plants having improved biomass digestibility
Christine Horlow, Paris (FR); Marie-Hélène Durand-Tardif, Paris (FR); Gregory Mouille, Chartres (FR); and Peter Rogowsky, Lyons (FR)
Assigned to Institut National de Recherche Pour l'Agriculture, l'Alimentation et l'Environnement, Paris (FR)
Appl. No. 17/427,992
Filed by Institut National de Recherche Pour l'Agriculture, L'Alimentation et L'Environnement, Paris (FR)
PCT Filed Feb. 7, 2020, PCT No. PCT/EP2020/053140 § 371(c)(1), (2) Date Aug. 3, 2021, PCT Pub. No. WO2020/161306, PCT Pub. Date Aug. 13, 2020.
Claims priority of application No. 1901260 (FR), filed on Feb. 8, 2019.
Prior Publication US 2022/0119835 A1, Apr. 21, 2022
Int. Cl. C12N 15/82 (2006.01); C12N 15/01 (2006.01)

CPC C12N 15/8255 (2013.01) [C12N 15/01 (2013.01)]

9 Claims

1. A plant obtained by a mutagenesis method comprising

decreasing the expression and/or the activity

of the protein designated ESK1 and

of the protein designated TPS7,

in said plant, as compared to a non-mutagenized parent plant, and the decrease in the expression and/or the activity of the ESK1 and TPS7 proteins is achieved by introducing at least one mutation in each of the genes encoding the ESK1 and TPS7 proteins in said plant, and such that:

the non-mutagenized ESK1 protein has at least 50% identity with the sequence SEQ ID NO: 1 and comprising the acyl esterase and transmembrane domains, and

the non-mutagenized TPS7 protein has at least 60% identity with the sequence SEQ ID NO: 2 and comprising the TPS and TPP domains.