US 11,898,140 B2
Hyperthermophilic aerobic fermentation inoculant prepared by using municipal sewage sludge and its method
Shungui Zhou, Fuzhou (CN); Zhi Chen, Fuzhou (CN); and Hanpeng Liao, Fuzhou (CN)
Filed by FUJIAN AGRICULTURE AND FORESTRY UNIVERSITY, Fuzhou (CN)
Filed on Mar. 22, 2020, as Appl. No. 16/826,261.
Application 16/826,261 is a continuation of application No. PCT/CN2018/073426, filed on Jan. 19, 2018.
Prior Publication US 2020/0224153 A1, Jul. 16, 2020
Int. Cl. C12N 1/20 (2006.01)
CPC C12N 1/20 (2013.01) [C12N 2500/10 (2013.01); C12N 2500/34 (2013.01)] 8 Claims
 
1. A preparation method for a hyperthermophilic aerobic fermentation inoculant prepared by using sewage sludge, comprising:
fermenting hyperthermophilic aerobic bacteria to obtain a fermentation broth,
removing supernatant from the fermentation broth,
mixing the fermentation broth with protective agent to obtain a mixture,
drying the mixture,
pulverizing the dried mixture, and
sieving the pulverized mixture for packing;
wherein a culture medium for the fermentation is prepared by:
taking sewage sludge and adding water to the sewage sludge to regulate a solid content of the sewage sludge;
adding antifoaming agents of polyethers, yeast powder, ammonium chloride, sucrose, soluble starch, and potassium dihydrogen phosphate to the sewage sludge;
regulating pH of the sewage sludge;
adding composite carrier into the sewage sludge, and stirring the sewage sludge for uniformly mixing the composite carrier and the sewage sludge;
sterilizing the mixture of the composite carrier and the sewage sludge; and
cooling the sewage sludge to obtain the culture medium for the fermentation.