US 11,898,138 B2
Tissue processing apparatus and method for processing adipose tissue
William W. Cimino, Louisville, CO (US); Ramon Llull, Palma de Mallorca (ES); and Adam J. Katz, Winston-Salem, NC (US)
Assigned to THE GID GROUP, INC., Louisville, CO (US)
Filed by GID Bio, Inc., Louisville, CO (US)
Filed on Dec. 8, 2022, as Appl. No. 18/077,947.
Application 16/422,737 is a division of application No. 14/915,867, granted, now 10,336,980, issued on Jul. 2, 2019, previously published as PCT/US2014/054373, filed on Sep. 5, 2014.
Application 18/077,947 is a continuation of application No. 16/422,737, filed on May 24, 2019, granted, now 11,649,427.
Claims priority of provisional application 61/926,148, filed on Jan. 10, 2014.
Claims priority of application No. PCT/US2013/058292 (WO), filed on Sep. 5, 2013; and application No. PCT/US2014/011152 (WO), filed on Jan. 10, 2014.
Prior Publication US 2023/0113828 A1, Apr. 13, 2023
Int. Cl. C12M 1/00 (2006.01); C12M 1/33 (2006.01); A61L 27/00 (2006.01); A61K 35/32 (2015.01); C12N 5/077 (2010.01); A61M 1/00 (2006.01)
CPC C12M 47/04 (2013.01) [A61K 35/32 (2013.01); A61L 27/00 (2013.01); C12M 45/02 (2013.01); C12M 45/05 (2013.01); C12N 5/0653 (2013.01); A61M 1/892 (2021.05); A61M 2202/08 (2013.01)] 31 Claims
OG exemplary drawing
 
1. A method for preparing a product for treatment of osteoarthritis in a human patient with uncultured stromal vascular fraction cells derived from harvested adipose tissue from the patient, the method comprising:
washing a volume of harvested adipose tissue from the patient within a single-use, portable processing container to remove blood components from the harvested adipose tissue and prepare washed adipose tissue;
after the washing, enzymatically digesting the washed adipose tissue within the processing container, the enzymatically digesting comprising combining the washed adipose tissue and an enzyme-containing digestion medium in a mixture and agitating the mixture for a digestion retention time to release stromal vascular fraction cells from the washed adipose tissue;
after the enzymatically digesting, centrifuging the processing container to form in the processing container a density-separated pellet phase of stromal vascular fraction cell concentrate comprising stromal vascular fraction cells released from the washed adipose tissue during the enzymatically digesting;
after the centrifuging, selectively removing at least a portion of the stromal vascular fraction cell concentrate from the processing container; and
disposing in a syringe an osteoarthritis treatment formulation comprising a quantity of the stromal vascular fraction cell concentrate removed from the processing container dispersed in a liquid dispersion medium, wherein the syringe is configured to administer by injection the osteoarthritis treatment formulation to the patient in one or more injections for treatment of the osteoarthritis at one or more joints of the patient; and
the osteoarthritis treatment formulation in the syringe comprises a volume of from 0.25 milliliter to 1.5 milliliters of the stromal vascular fraction cell concentrate.