US 11,898,132 B2
Bioreactor and method of forming complex three-dimensional tissue constructs
Lisa M. Larkin, Ann Arbor, MI (US); Ellen M. Arruda, Ann Arbor, MI (US); Michael J. Smietana, Ann Arbor, MI (US); and Pablo Moncada-Larrotiz, Ann Arbor, MI (US)
Assigned to The Regents of the University of Michigan, Ann Arbor, MI (US)
Filed by The Regents of the University of Michigan, Ann Arbor, MI (US)
Filed on Jul. 20, 2021, as Appl. No. 17/380,833.
Application 17/380,833 is a continuation of application No. 16/197,944, filed on Nov. 21, 2018, granted, now 11,078,454.
Application 16/197,944 is a continuation of application No. 15/211,501, filed on Jul. 15, 2016, granted, now 10,167,444, issued on Jan. 1, 2019.
Claims priority of provisional application 62/193,030, filed on Jul. 15, 2015.
Prior Publication US 2021/0348105 A1, Nov. 11, 2021
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/12 (2006.01); C12M 1/00 (2006.01); C12M 1/24 (2006.01); C12M 1/42 (2006.01)
CPC C12M 25/14 (2013.01) [C12M 23/04 (2013.01); C12M 23/08 (2013.01); C12M 23/34 (2013.01); C12M 23/50 (2013.01); C12M 29/10 (2013.01); C12M 35/08 (2013.01)] 21 Claims
OG exemplary drawing
 
1. A bioreactor for forming a complex three-dimensional tissue construct, comprising:
a first substrate for culturing a first cell source;
a second substrate for culturing a second cell source, wherein the first substrate and the second substrate comprise two walls of a culture chamber; and
at least one flexible or rigid post in the culture chamber that at least partially extends between the first substrate and the second substrate so that a first tissue construct of the first cell source cultured on the first substrate or a second tissue construct of the second cell source cultured on the second substrate can be translated via the flexible or rigid post to form the complex three-dimensional tissue construct having the second tissue construct of the second cell source cultured with the first tissue construct of the first cell source.