US 11,897,951 B2
Polypeptides comprising immunoglobulin single variable domains targeting IL-6 and TNF-α
Heidi Rommelaere, Ghent (BE); Christian Asbrand, Frankfurt am Main (DE); Nadine Biesemann, Zwijnaarde (BE); Ann Brigé, Ertvelde (BE); Sigrid Cornelis, St. Martens-Lataem (BE); Karen Heyninck, Zwijnaarde (BE); Eric Lorent, Zwijnaarde (BE); and Shanna Van Zwam, Ghent (BE)
Assigned to Ablynx N.V., Zwijnaarde (BE); and Sanofi, Paris (FR)
Filed by Ablynx N.V., Zwijnaarde (BE); and Sanofi, Paris (FR)
Filed on Dec. 17, 2021, as Appl. No. 17/553,916.
Claims priority of provisional application 63/167,925, filed on Mar. 30, 2021.
Claims priority of application No. 20 306 612 (EP), filed on Dec. 18, 2020.
Prior Publication US 2022/0356241 A1, Nov. 10, 2022
Int. Cl. C07K 16/24 (2006.01); A61P 29/00 (2006.01); C07K 16/28 (2006.01)
CPC C07K 16/248 (2013.01) [A61P 29/00 (2018.01); C07K 16/2875 (2013.01); C07K 2317/31 (2013.01); C07K 2317/565 (2013.01); C07K 2317/94 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A polypeptide, or a composition comprising the polypeptide, wherein the polypeptide comprises at least three immunoglobulin single variable domains (ISVDs), wherein each of said ISVDs comprises three complementarity determining regions (CDR1 to CDR3, respectively), optionally linked via one or more peptidic linkers; and wherein:
a) a first ISVD binds to IL-6 and comprises
i. a CDR1 which has the amino acid sequence of SEQ ID NO: 6;
ii. a CDR2 which has the amino acid sequence of SEQ ID NO: 10 amino acid difference(s) with SEQ ID NO: 10; and
iii. a CDR3 which has the amino acid sequence of SEQ ID NO: 14;
b) a second ISVD binds to IL-6 and comprises;
iv. a CDR1 which has the amino acid sequence of SEQ ID NO: 8
v. a CDR2 which has the amino acid sequence of SEQ ID NO: 12 amino acid difference(s) with SEQ ID NO: 12; and
vi. a CDR3 which has the amino acid sequence of SEQ ID NO: 16; and
c) a third ISVD binds to TNF-α and comprises
vii. a CDR1 which has the amino acid sequence of SEQ ID NO: 9;
viii. a CDR2 which has the amino acid sequence of SEQ ID NO: 13 amino acid difference(s) with SEQ ID NO: 13; and
ix. a CDR3 which has the amino acid sequence of SEQ ID NO: 17.