US 11,897,929 B2
Compositions and methods for targeted cytokine delivery
Alexander Sasha Krupnick, St. Louis, MO (US); Daved Henry Fremont, St. Louis, MO (US); Eric Reed Lazear, St. Louis, MO (US); and John Westwick, Houston, TX (US)
Assigned to Washington University, St. Louis, MO (US)
Filed by Washington University, St. Louis, MO (US)
Filed on Apr. 23, 2021, as Appl. No. 17/238,995.
Application 17/238,995 is a continuation of application No. 16/075,069, granted, now 11,053,293, previously published as PCT/US2017/016688, filed on Feb. 6, 2017.
Claims priority of provisional application 62/419,146, filed on Nov. 8, 2016.
Claims priority of provisional application 62/350,056, filed on Jun. 14, 2016.
Claims priority of provisional application 62/342,630, filed on May 27, 2016.
Claims priority of provisional application 62/292,046, filed on Feb. 5, 2016.
Prior Publication US 2021/0284706 A1, Sep. 16, 2021
Int. Cl. C07K 14/55 (2006.01); A61K 47/68 (2017.01); A61P 35/00 (2006.01); C07K 16/28 (2006.01); A61K 38/00 (2006.01); C07K 14/54 (2006.01); C07K 14/545 (2006.01); C07K 14/705 (2006.01); C07K 14/52 (2006.01)
CPC C07K 14/55 (2013.01) [A61K 47/6849 (2017.08); A61P 35/00 (2018.01); C07K 14/52 (2013.01); C07K 14/545 (2013.01); C07K 14/5418 (2013.01); C07K 14/5443 (2013.01); C07K 14/70596 (2013.01); C07K 16/2851 (2013.01); A61K 38/00 (2013.01)] 5 Claims
 
1. A method of expanding cytotoxic lymphocytes ex vivo, comprising culturing a population of lymphocytes in the presence of a chimeric peptide,
wherein the chimeric peptide comprises an orthopoxvirus major histocompatibility complex class 1-like protein (OMCP) peptide linked to an interleukin-2 (IL-2) mutant peptide comprising the amino acid sequence of SEQ ID NO: 5 comprising at least one mutation, wherein the at least one mutation is R38A, F42K, or C125S relative to SEQ ID NO: 5 wherein the OMCP peptide comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 7, SEQ ID NO: 13, and SEQ ID NO: 14, and
wherein the population of lymphocytes comprises cytotoxic lymphocytes.