	US 11,897,918 B2
	Compositions and methods to increase production
Samuel Ho, Cambridge, MA (US); David Parker, Cambridge, MA (US); Peter Fekkes, Cambridge, MA (US); Ivna De Souza, Cambridge, MA (US); Peter Mason, Cambridge, MA (US); and Pirada Allen, Cambridge, MA (US)
Assigned to Seqirus UK Limited, Berkshire (GB)
Filed by Seqirus UK Limited, Berkshire (GB)
Filed on Mar. 11, 2019, as Appl. No. 16/297,895.
Application 16/297,895 is a continuation of application No. 15/304,650, abandoned, previously published as PCT/EP2015/058533, filed on Apr. 20, 2015.
Claims priority of application No. 14165334 (EP), filed on Apr. 18, 2014.
Prior Publication US 2020/0040040 A1, Feb. 6, 2020
Int. Cl. C07K 14/005 (2006.01); A61K 39/12 (2006.01); A61K 39/145 (2006.01); C12N 7/02 (2006.01); C12N 7/00 (2006.01); A61K 39/00 (2006.01)

CPC C07K 14/005 (2013.01) [A61K 39/12 (2013.01); A61K 39/145 (2013.01); C12N 7/00 (2013.01); C12N 7/02 (2013.01); A61K 39/00 (2013.01); A61K 2039/575 (2013.01); C12N 2760/16111 (2013.01); C12N 2760/16134 (2013.01); C12N 2760/16143 (2013.01); C12N 2760/16151 (2013.01); C12N 2760/16234 (2013.01); C12N 2760/16243 (2013.01); C12N 2760/16251 (2013.01)]

14 Claims

1. A method for producing influenza virus particles and influenza virus proteins from suspension MDCK cells comprising:

growing or culturing the suspension MDCK cells for a duration of time, at least a portion of which is carried out in the presence of at least one chemical agent selected from the group consisting of fluvastatin, atorvastatin, cerivastatin, lovastatin, mevastatin, pravastatin or isomers thereof;

harvesting the influenza virus particles and influenza virus proteins from the suspension MDCK cells; and

formulating the influenza virus particles and influenza virus proteins into a pharmaceutical composition;

wherein the at least one chemical agent has a concentration of 0.001 μM to 5 μM in culture.