	US 11,896,969 B2
	Anionic exchange-hydrophobic mixed mode chromatography resins
Christopher Belisle, Walnut Creek, CA (US); Xuemei He, Walnut Creek, CA (US); Hong Chen, San Ramon, CA (US); Yueping Xu, Albany, CA (US); and Jiali Liao, San Ramon, CA (US)
Assigned to BIO-RAD LABORATORIES, INC., Hercules, CA (US)
Filed by BIO-RAD LABORATORIES, INC., Hercules, CA (US)
Filed on Sep. 4, 2020, as Appl. No. 17/012,108.
Claims priority of provisional application 62/896,196, filed on Sep. 5, 2019.
Prior Publication US 2021/0069692 A1, Mar. 11, 2021
Int. Cl. B01J 41/07 (2017.01); B01J 47/02 (2017.01); C07K 1/18 (2006.01); C12N 9/88 (2006.01); B01J 47/014 (2017.01); B01J 41/14 (2006.01)

CPC B01J 41/14 (2013.01) [B01J 41/07 (2017.01); B01J 47/014 (2017.01); B01J 47/02 (2013.01); C07K 1/18 (2013.01); C12N 9/88 (2013.01); C12Y 402/01045 (2013.01); B01J 2220/52 (2013.01)]

12 Claims

1. A chromatography matrix covalently linked to a ligand having the following formula:

Chromatography matrix-(X)—N(R¹)—(R²-L)_n-Ar

or an anionic salt thereof,

wherein:

X is a spacer;

R¹is hydrogen or C₁to C₆alkyl optionally substituted with an —OH;

R²is C₂to C₆alkyl or C₄to C₆cycloalkyl;

L is NR⁴, O, or S;

n=1 or 2; and

Ar is a 6-10 membered ring and:

if Ar is aryl, the aryl is optionally substituted with up to five C₁to C₃unsubstituted alkyl, C₃to C₆branched alkyl, unsubstituted aryl, or fluorine groups; or

if Ar is heteroaryl, the heteroaryl is optionally substituted with up to four unsubstituted alkyl groups,

with the proviso that when R¹is hydrogen, R²is C₂alkyl, L is NR⁴or O, and n is 1, Ar is not phenyl.