US 11,896,944 B2
System and method with fiducials responding to multiple excitation frequencies
John S. Vieceli, Encinitas, CA (US); Alex Nemiroski, San Diego, CA (US); Paul Belitz, San Diego, CA (US); Robert Langlois, San Diego, CA (US); M. Shane Bowen, Encinitas, CA (US); Danny Yuan Chan, San Diego, CA (US); Bala Murali K. Venkatesan, San Francisco, CA (US); Hui Han, San Diego, CA (US); Kevan Samiee, San Diego, CA (US); and Stephen Tanner, San Diego, CA (US)
Assigned to Illumina, Inc., San Diego, CA (US)
Appl. No. 16/482,592
Filed by Illumina, Inc., San Diego, CA (US)
PCT Filed Jan. 31, 2018, PCT No. PCT/US2018/016132
§ 371(c)(1), (2) Date Jul. 31, 2019,
PCT Pub. No. WO2018/144531, PCT Pub. Date Aug. 9, 2018.
Claims priority of application No. 1701684 (GB), filed on Feb. 1, 2017; application No. 1701685 (GB), filed on Feb. 1, 2017; and application No. 1701690 (GB), filed on Feb. 1, 2017.
Prior Publication US 2020/0009525 A1, Jan. 9, 2020
Int. Cl. C12Q 1/6837 (2018.01); B01J 19/00 (2006.01); G01N 21/64 (2006.01); G06T 7/00 (2017.01)
CPC B01J 19/0046 (2013.01) [C12Q 1/6837 (2013.01); G01N 21/6456 (2013.01); G06T 7/0012 (2013.01); B01J 2219/00576 (2013.01); B01J 2219/00608 (2013.01); B01J 2219/00722 (2013.01)] 22 Claims
 
1. An array, comprising:
a support having a plurality of areas, each of the plurality of areas having locations that, in operation, receive biological samples differing from one another to respond differently in successive cycles of fluorescent imaging; and
a fiducial feature on the support in each of the plurality of areas, each of the fiducial features including at least one depression and a plurality of autofluorescent beads disposed in the at least one depression, the at least one depression having a shape that separates an inner area where some of the locations are located and an outer area where some other of the locations are located, the shape being selected from the group consisting of a single circle, a single segmented circle, two non-concentric and segmented rings, and a non-closed shape, the autofluorescent beads being formed of a fluorescent material that responds by producing a detectable signal in the successive cycles of fluorescent imaging.