US 11,896,654 B2
DNA monoclonal antibodies targeting checkpoint molecules
David B. Weiner, Merion, PA (US); Kar Muthumani, Cherry Hill, NJ (US); and Niranjan Sardesai, Blue Bell, PA (US)
Assigned to The Trustees of the University of Pennsylvania, Philadelphia, PA (US); The Wistar Institute of Anatomy and Biology, Philadelphia, PA (US); and Inovio Pharmaceuticals, Plymouth Meeting, PA (US)
Appl. No. 16/098,925
Filed by The Trustees of the University of Pennsylvania, Philadelphia, PA (US); THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY, Philadelphia, PA (US); and Inovio Pharmaceuticals, Inc., Plymouth Meeting, PA (US)
PCT Filed May 5, 2017, PCT No. PCT/US2017/031440
§ 371(c)(1), (2) Date Nov. 5, 2018,
PCT Pub. No. WO2017/193094, PCT Pub. Date Nov. 9, 2017.
Claims priority of provisional application 62/332,386, filed on May 5, 2016.
Prior Publication US 2019/0142920 A1, May 16, 2019
Int. Cl. A61K 39/00 (2006.01); C12N 15/85 (2006.01); C07K 16/28 (2006.01); A61K 31/7088 (2006.01); A61P 35/00 (2006.01); A61K 39/395 (2006.01)
CPC A61K 39/001129 (2018.08) [A61K 31/7088 (2013.01); A61K 39/395 (2013.01); A61P 35/00 (2018.01); C07K 16/2803 (2013.01); C07K 16/2818 (2013.01); C07K 16/2878 (2013.01); C12N 15/85 (2013.01); A61K 2039/505 (2013.01); A61K 2039/53 (2013.01)] 16 Claims
 
1. A composition for generating a synthetic antibody in a subject comprising one or more nucleic acid molecules encoding one or more antibodies or fragments thereof, wherein the one or more antibodies or fragments target at least one immune checkpoint molecule selected from the group consisting of PD-1, LAG-3, PD-L1, GITR, CD40, OX40, CTLA-4, TIM-3, 4-1BB, and a combination thereof,
wherein the one or more nucleic acid molecules comprises a nucleotide sequence encoding an amino acid sequence having at least about 80% identity over the entire length of an amino acid sequence selected from the group consisting of SEQ ID NOs: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, and 28, wherein said amino acid sequence comprises heavy and light chain CDR amino acid sequences selected from the group consisting of SEQ ID Nos: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, and 28.