US 11,896,030 B2
Tobacco-derived protein compositions
Anthony Richard Gerardi, Winston-Salem, NC (US); Crystal Dawn Hege Byrd, Lexington, NC (US); Thaddeus Jude Jackson, High Point, NC (US); Chelsea Allison Cooke, Winston-Salem, NC (US); John-Paul Mua, Advance, NC (US); and Kyle Ford, Germantown, NC (US)
Assigned to R.J. Reynolds Tobacco Company, Winston-Salem, NC (US)
Filed by R.J. REYNOLDS TOBACCO COMPANY, Winston-Salem, NC (US)
Filed on Oct. 8, 2015, as Appl. No. 14/878,561.
Application 14/878,561 is a division of application No. 13/896,656, filed on May 17, 2013, granted, now 9,175,052.
Prior Publication US 2016/0029663 A1, Feb. 4, 2016
This patent is subject to a terminal disclaimer.
Int. Cl. A23J 1/00 (2006.01); C07K 14/415 (2006.01); A23J 3/14 (2006.01); A23L 2/66 (2006.01); A24B 15/18 (2006.01); A24B 15/24 (2006.01); A23K 20/147 (2016.01); A23L 5/20 (2016.01); A23L 33/185 (2016.01); A24B 15/20 (2006.01); C12N 9/88 (2006.01)
CPC A23J 1/007 (2013.01) [A23J 3/14 (2013.01); A23K 20/147 (2016.05); A23L 2/66 (2013.01); A23L 5/23 (2016.08); A23L 5/273 (2016.08); A23L 33/185 (2016.08); A24B 15/18 (2013.01); A24B 15/20 (2013.01); A24B 15/24 (2013.01); A24B 15/241 (2013.01); C07K 14/415 (2013.01); C12N 9/88 (2013.01); C12Y 401/01039 (2013.01); A23V 2002/00 (2013.01)] 20 Claims
 
1. A method for modifying the content of a plant-derived, protein-enriched material, the method comprising
receiving a plant-derived, protein-enriched material comprising RuBisCO, F2 fraction proteins, or a combination thereof, wherein the plant-derived, protein-enriched material further comprises one or more heavy metals;
dissolving at least a portion of the plant-derived, protein-enriched material in a solvent to form a solution,
contacting the plant-derived, protein-enriched material solution with a functionalized resin to afford a treated solution comprising a protein concentrate or isolate having a reduced amount of one or more of copper, cadmium, lead, and nickel as compared with the plant-derived, protein-enriched material,
wherein the functionalized resin comprises a first resin functionalized with triaminetetraacetic acid chelating groups, and a second resin functionalized with triaminetetraacetate, sodium salt chelating groups;
separating the treated solution from the functionalized resin,
wherein the contacting step is conducted for a period of 1 to 3 hours; and
wherein the contacting step comprises agitating the plant-derived, protein-enriched material solution with the functionalized resin at room temperature, and/or
heating the plant-derived, protein-enriched material solution at a temperature of 50° C. to 70° C.