US 11,896,012 B2
Phitosterols suspension concentrates and process of preparation thereof
Paolo Bellandi, Carcare (IT); Ilenia Fiorenza Mazzali, Novara (IT); Elisa Galimberti, Lissone (IT); and Claudio Dacarro, Milan (IT)
Assigned to ISAGRO S.P.A., Milan (IT)
Filed by ISAGRO S.P.A., Milan (IT)
Filed on Feb. 24, 2022, as Appl. No. 17/679,997.
Application 17/679,997 is a division of application No. 16/618,870, granted, now 11,291,207, previously published as PCT/IB2018/054385, filed on Jun. 14, 2018.
Claims priority of application No. 102017000066508 (IT), filed on Jun. 15, 2017.
Prior Publication US 2022/0295794 A1, Sep. 22, 2022
This patent is subject to a terminal disclaimer.
Int. Cl. A01N 45/00 (2006.01); A01N 25/04 (2006.01)
CPC A01N 45/00 (2013.01) [A01N 25/04 (2013.01)] 15 Claims
 
1. A method of stimulating plant growth comprising:
a) preparing a concentrated suspension containing phytosterols, comprising grinding one or more phytosterols until an average particle size of less than 10 pm is reached, said concentrated suspension comprising:
i) a phytosterol mixture of beta-sitosterol, stigmasterol, cam pesterol and brassicasterol, in an amount higher than 25% by weight with respect to the total weight of the concentrated suspension, wherein beta-sitosterol is present in an amount ranging from 40% to 45% by weight with respect to the total weight of the mixture of phytosterols, the campesterol is present in an amount ranging from 20% to 25% by weight with respect to the total weight of the mixture of phytosterols, the stigmasterol is present in an amount ranging from 20% to 30% by weight with respect to the total weight of the mixture of phytosterols and the brassicasterol is present in an amount ranging from 1% to 5% by weight with respect to the total weight of the mixture of phytosterols;
ii) one or more wetting agents in an amount ranging from 1% to 5% by weight with respect to the total weight of the concentrated suspension; and
iii) one or more dispersing agents in an amount ranging from 1% to 5% by weight with respect to the total weight of the concentrated suspension;
b) applying the concentrated suspension by one or more of application to any part of a plant, application to the soil by means of fertigation, incorporation into the ground, through seed tanning, and by combinations thereof.