US 11,895,962 B2
Method for producing agave cultures for tequila
Peter Andrew Whitton, Long Beach, CA (US); John R. Munoz, Long Beach, CA (US); and Geoffrey Dixon, Long Beach, CA (US)
Assigned to PHYTOTEQ, INC., Long Beach, CA (US)
Filed by PHYTOTEQ, INC., Long Beach, CA (US)
Filed on Jun. 29, 2022, as Appl. No. 17/853,163.
Claims priority of provisional application 63/312,789, filed on Feb. 22, 2022.
Prior Publication US 2023/0263120 A1, Aug. 24, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12G 3/02 (2019.01); A01H 4/00 (2006.01); C12N 5/04 (2006.01)
CPC A01H 4/005 (2013.01) [C12G 3/02 (2013.01); C12N 5/04 (2013.01)] 10 Claims
 
1. A method for cultivating agave cells for the product of tequila, comprising:
(a) prepare a callus introduction media including sucrose, naphthalene acetic acid, and water;
(b) place agave cells into the introduction media to incubate a callus formation;
(c) establish a mixture by placing the callus formation into an accelerator media including sucrose, naphthalene acetic acid, and water, supplemented by a vitamin stock solution, and modify the mixture to obtain a pH of between 5.5 and 6;
(d) heat the mixture of (c);
(e) use cultures obtained from the mixture of (d) to cultivate subcultures from accelerator media and supplement with citric acid to obtain a pH of between 3.5 and 4.5, and introduce light to the subcultures;
(f) add carbon dioxide and yeast, and ferment;
whereupon an ethanol concentration is adjusted to produce tequila.