US 12,224,154 B2
Conductive fixation for electron microscopy
Eduardo Rosa-Molinar, Lawrence, KS (US)
Assigned to University of Kansas, Lawrence, KS (US)
Appl. No. 16/961,328
Filed by UNIVERSITY OF KANSAS, Lawrence, KS (US)
PCT Filed Jan. 10, 2019, PCT No. PCT/US2019/013051
§ 371(c)(1), (2) Date Jul. 10, 2020,
PCT Pub. No. WO2019/140089, PCT Pub. Date Jul. 18, 2019.
Claims priority of provisional application 62/615,811, filed on Jan. 10, 2018.
Claims priority of provisional application 62/615,866, filed on Jan. 10, 2018.
Prior Publication US 2020/0373121 A1, Nov. 26, 2020
Int. Cl. H01J 37/00 (2006.01); C08L 101/12 (2006.01); G01N 1/30 (2006.01); G01N 1/42 (2006.01); G01Q 30/02 (2010.01); H01J 37/26 (2006.01)
CPC H01J 37/268 (2013.01) [C08L 101/12 (2013.01); G01N 1/30 (2013.01); G01N 1/42 (2013.01); G01Q 30/02 (2013.01); G01N 2001/302 (2013.01); G01N 2001/305 (2013.01)] 20 Claims
OG exemplary drawing
 
1. A method of preparing a biological sample for microanalysis, the method comprising:
(i) contacting the biological sample with a freeze substitution solution comprising a lithium salt under conditions effective to permit permeation of the lithium salt into the biological sample;
(ii) contacting the biological sample with a first polymerizable resin under conditions effective to permit permeation of the polymerizable resin into the biological sample;
(iii) disposing the biological sample in a volume of a second polymerizable resin; and
(iv) heating the so-prepared biological sample to a temperature effective to cure the first polymerizable resin and the second polymerizable resin, thereby forming a 3-dimensional conductive sample block;
wherein the lithium salt is chosen from lithium trifluoromethanesulfonate, lithium carbonate, lithium hydroxide, lithium citrate, lithium nitrate, lithium benzoate, lithium formate, and combinations thereof.