US 12,223,651 B2
Identifying nucleotides by determining phasing
Francisco Jose Garcia, San Diego, CA (US); Klaus Maisinger, Essex (GB); Stephen Tanner, San Diego, CA (US); John A. Moon, San Diego, CA (US); Tobias Mann, San Diego, CA (US); Michael Lawrence Parkinson, Reyrieux (FR); Anthony James Cox, Essex (GB); and Haifang H. Ge, Essex (GB)
Assigned to Illumina, Inc., San Diego, CA (US)
Filed by Illumina, Inc., San Diego, CA (US)
Filed on Apr. 19, 2023, as Appl. No. 18/303,436.
Application 18/303,436 is a continuation of application No. 17/445,994, filed on Aug. 26, 2021, granted, now 11,676,275.
Application 17/445,994 is a continuation of application No. 17/157,622, filed on Jan. 25, 2021, granted, now 11,605,165, issued on Mar. 14, 2023.
Application 17/157,622 is a continuation of application No. 16/378,894, filed on Apr. 9, 2019, abandoned.
Application 16/378,894 is a continuation of application No. 15/354,540, filed on Nov. 17, 2016, granted, now 10,304,189, issued on May 28, 2019.
Application 15/354,540 is a continuation of application No. 14/608,471, filed on Jan. 29, 2015, granted, now 9,530,207, issued on Dec. 27, 2016.
Application 14/608,471 is a continuation of application No. 13/006,206, filed on Jan. 13, 2011, granted, now 8,965,076, issued on Feb. 24, 2015.
Claims priority of provisional application 61/321,029, filed on Apr. 5, 2010.
Claims priority of provisional application 61/294,811, filed on Jan. 13, 2010.
Prior Publication US 2023/0351598 A1, Nov. 2, 2023
Int. Cl. G06T 7/00 (2017.01); G06T 7/246 (2017.01); G06T 7/33 (2017.01); G06T 7/90 (2017.01)
CPC G06T 7/0014 (2013.01) [G06T 7/0012 (2013.01); G06T 7/246 (2017.01); G06T 7/248 (2017.01); G06T 7/33 (2017.01); G06T 7/337 (2017.01); G06T 7/90 (2017.01); G06T 2207/10024 (2013.01); G06T 2207/30024 (2013.01); G06T 2207/30072 (2013.01)] 18 Claims
OG exemplary drawing
 
1. A method for creating a template of nucleic acid cluster locations on a flow cell, comprising:
obtaining fluorescent emission signals from nucleic acid clusters on the flow cell, wherein a nucleic acid cluster comprises a plurality of nucleic acid molecules attached to the flow cell, and wherein the plurality of the nucleic acid molecules comprise fluorescently labeled nucleotides;
identifying a plurality of candidate cluster locations on the flowcell from the obtained fluorescent emission signals; and
determining whether to keep a candidate cluster location, discard a candidate cluster location, or merge a candidate cluster location with another location based on the obtained fluorescent emission signals, thereby generating a template of cluster locations on the flow cell.