	US 12,222,349 B2
	Compositions, methods and systems for protein corona analysis and uses thereof
Hongwei Xia, Fremont, CA (US); Lyndal Hesterberg, Loveland, CO (US); Michael Figa, San Mateo, CA (US); Xiaoyan Zhao, Foster City, CA (US); Gregory Troiano, Pembroke, MA (US); William Manning, Redwood City, CA (US); John Blume, Bellingham, WA (US); Omid Farokhzad, Waban, MA (US); Matthew McLean, Redwood City, CA (US); Craig Stolarczyk, San Mateo, CA (US); Marwin Ko, Redwood City, CA (US); and Theodore Platt, Danville, CA (US)
Assigned to Seer, Inc., Redwood City, CA (US)
Filed by Seer, Inc., Redwood City, CA (US)
Filed on Mar. 29, 2021, as Appl. No. 17/216,514.
Application 17/216,514 is a continuation of application No. PCT/US2019/000061, filed on Nov. 7, 2019.
Claims priority of provisional application 62/756,960, filed on Nov. 7, 2018.
Claims priority of provisional application 62/824,278, filed on Mar. 26, 2019.
Claims priority of provisional application 62/874,862, filed on Jul. 16, 2019.
Prior Publication US 2021/0215685 A1, Jul. 15, 2021
Int. Cl. G01N 33/543 (2006.01); G01N 33/551 (2006.01); G01N 33/68 (2006.01)

CPC G01N 33/54326 (2013.01) [G01N 33/54346 (2013.01); G01N 33/551 (2013.01); G01N 33/6848 (2013.01)]

36 Claims

1. A kit for identifying proteins in a biological sample, comprising:

(a) a plurality of magnetic particles configured to bind a plurality of proteins in the biological sample when the plurality of magnetic particles is incubated with the biological sample, wherein the plurality of magnetic particles comprise a polymer, wherein the polymer comprises an ammonium functional group, wherein the polymer comprises a recurring unit of:

wherein the plurality of magnetic particles comprise a positive zeta potential, and wherein the positive zeta potential of the plurality of magnetic particles range from 10 mV to 60 mV;

(b) a reducing agent configured to reduce the plurality of proteins; and