US 12,222,345 B2
System for imaging captured cells
Kalyan Handique, Ann Arbor, MI (US); Kyle Gleason, Brighton, MI (US); Christopher Siemer, Ypsilanti, MI (US); Priyadarshini Gogoi, Ann Arbor, MI (US); Saedeh Sepehri Javdani, Ypsilanti, MI (US); Gene Parunak, Plymouth, MI (US); Aaron Kehrer, Plymouth, MI (US); and Jon Meines, Plymouth, MI (US)
Assigned to BIO-RAD LABORATORIES, INC., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on May 14, 2020, as Appl. No. 15/931,928.
Application 15/931,928 is a continuation of application No. 16/589,778, filed on Oct. 1, 2019, granted, now 10,690,650.
Application 16/589,778 is a continuation of application No. 15/430,833, filed on Feb. 13, 2017, granted, now 10,509,022, issued on Dec. 17, 2019.
Application 15/430,833 is a continuation of application No. 15/199,245, filed on Jun. 30, 2016, granted, now 9,612,199, issued on Apr. 4, 2017.
Application 15/199,245 is a continuation of application No. 14/208,458, filed on Mar. 13, 2014, granted, now 9,404,864, issued on Aug. 2, 2016.
Claims priority of provisional application 61/902,431, filed on Nov. 11, 2013.
Claims priority of provisional application 61/779,090, filed on Mar. 13, 2013.
Prior Publication US 2020/0271637 A1, Aug. 27, 2020
Int. Cl. G01N 33/483 (2006.01); G01N 21/25 (2006.01); G01N 21/64 (2006.01); G01N 21/76 (2006.01); G01N 33/487 (2006.01); G02B 21/06 (2006.01); G02B 21/26 (2006.01); A61B 5/00 (2006.01)
CPC G01N 33/4833 (2013.01) [G01N 21/253 (2013.01); G01N 21/6428 (2013.01); G01N 21/6458 (2013.01); G01N 21/6486 (2013.01); G01N 21/76 (2013.01); G01N 33/48735 (2013.01); G02B 21/06 (2013.01); G02B 21/26 (2013.01); A61B 5/00 (2013.01); G01N 2021/6439 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method comprising:
receiving an imaging substrate at a platform positioned between: a first light source configured to transmit light toward the imaging substrate from a first direction and a second light source configured to transmit light toward the imaging substrate from a second direction different from the first direction; and
generating an image dataset from the imaging substrate, by way of a lens actuator coupled to a lens configured to transmit light from a target object toward an optical sensor, the target object comprising a tag having one or more features coupled to the imaging substrate, wherein the tag is defined at a surface of the imaging substrate and,
wherein generating the image dataset comprises:
actuating the platform relative to a first axis perpendicular to a plane defined by the platform, into a first configuration that aligns the imaging substrate into alignment with the first light source, the second light source, and the lens, and
adjusting operation of the lens actuator, thereby automatically focusing the lens relative to the target object by way of the tag.