US 12,222,334 B2
Use of low-bind surface coatings for analysis of tyrosine kinase inhibitors
Stephen Balloch, Stockport (GB); Lisa Jane Calton, Stockport (GB); and Gareth Hammond, Stockport (GB)
Assigned to Micromass UK Limited, Wilmslow (GB)
Filed by Micromass UK Limited, Wilmslow (GB)
Filed on Apr. 27, 2022, as Appl. No. 17/730,271.
Claims priority of provisional application 63/180,364, filed on Apr. 27, 2021.
Prior Publication US 2022/0341896 A1, Oct. 27, 2022
Int. Cl. G01N 30/16 (2006.01); G01N 30/72 (2006.01); G01N 33/94 (2006.01); G01N 30/02 (2006.01)
CPC G01N 30/16 (2013.01) [G01N 30/7233 (2013.01); G01N 33/94 (2013.01); G01N 2030/027 (2013.01)] 14 Claims
OG exemplary drawing
 
1. A method of separating and analyzing tyrosine kinase inhibitors, the method comprising:
injecting a sample comprising one or more tyrosine kinase inhibitors into a chromatographic system, wherein the sample is prepared from plasma or serum obtained from a subject, and wherein the chromatographic system comprises a metallic flow path, wherein at least a portion of the metallic flow path is coated with a low-bind surface coating;
flowing the sample through the chromatographic system;
separating the one or more tyrosine kinase inhibitors from other components in the plasma or serum; and
passing the separated tyrosine kinase inhibitors to a detector.