US 12,221,655 B2
Pre-surgical risk stratification based on PDE4D7 and DHX9 expression
George Baillie, Glasgow (GB); and Ralf Dieter Hoffmann, Eindhoven (NL)
Assigned to Koninklijke Philips N.V., Eindhoven (NL)
Appl. No. 17/273,568
Filed by KONINKLIJKE PHILIPS N.V., Eindhoven (NL); and THE UNIVERSITY COURT OF THE UNIVERSITY OF GLASGOW, Glasgow (GB)
PCT Filed Oct. 11, 2019, PCT No. PCT/EP2019/077549
§ 371(c)(1), (2) Date Mar. 4, 2021,
PCT Pub. No. WO2020/074679, PCT Pub. Date Apr. 16, 2020.
Claims priority of application No. 18199784 (EP), filed on Oct. 11, 2018.
Prior Publication US 2021/0355543 A1, Nov. 18, 2021
Int. Cl. C12Q 1/6886 (2018.01)
CPC C12Q 1/6886 (2013.01) [C12Q 2600/112 (2013.01); C12Q 2600/158 (2013.01)] 8 Claims
 
1. A method of pre-surgical risk stratification of a prostate cancer subject, prior to the prostate cancer subject undergoing any prostate surgery, comprising:
determining a gene expression profile for phosphodiesterase 4D variant 7 (PDE4D7) in a biological sample obtained from the prostate cancer subject;
determining a gene expression profile for DExH-box helicase 9 (DHX9) in the same or another biological sample obtained from the prostate cancer subject; and
determining a pre-surgical prognostic risk score for the prostate cancer subject based on the gene expression profile for PDE4D7 and the gene expression profile for DHX9, wherein the determined pre-surgical prognostic risk score for the subject stratifies the prostate cancer subject into a high risk group comprising a high risk of disease progression for the prostate cancer subject; and
administering a primary treatment for the prostate cancer subject based on the pre-surgical prognostic risk score stratifying the prostate cancer subject into a high risk group comprising a high risk of disease progression for the prostate cancer subject, wherein the primary treatment is selected from the group consisting of: (i) at least a partial prostatectomy; (ii) an active therapy selected from radiation treatment, hormone therapy, chemotherapy; and (iii) a combination thereof.