Methods for simultaneous amplification of target loci
Joshua Babiarz, Castro Valley, CA (US); Tudor Pompiliu Constantin, Berkeley, CA (US); Lane A. Eubank, San Carlos, CA (US); George Gemelos, Portland, OR (US); Matthew Micah Hill, Belmont, CA (US); Huseyin Eser Kirkizlar, Los Angeles, CA (US); Matthew Rabinowitz, San Francisco, CA (US); Onur Sakarya, Redwood City, CA (US); Styrmir Sigurjonsson, San Jose, CA (US); and Bernhard Zimmermann, Manteca, CA (US)
Assigned to Natera, Inc., San Carlos, CA (US)
Filed by Natera, Inc., San Carlos, CA (US)
Filed on Mar. 3, 2022, as Appl. No. 17/685,730.
Application 17/685,730 is a continuation of application No. 16/747,833, filed on Jan. 21, 2020, granted, now 11,312,996.
Application 16/747,833 is a continuation of application No. 16/288,022, filed on Feb. 27, 2019, granted, now 10,597,723, issued on Mar. 24, 2020.
Application 16/288,022 is a continuation of application No. 16/140,298, filed on Sep. 24, 2018, abandoned.
Application 16/140,298 is a continuation of application No. 14/918,544, filed on Oct. 20, 2015, granted, now 10,316,362, issued on Jun. 11, 2019.
Application 14/918,544 is a continuation in part of application No. 14/877,925, filed on Oct. 7, 2015, abandoned.
Application 14/918,544 is a continuation in part of application No. 14/692,703, filed on Apr. 21, 2015, granted, now 10,179,937, issued on Jan. 15, 2019.
Application 14/918,544 is a continuation in part of application No. 14/538,982, filed on Nov. 24, 2014, granted, now 9,677,118, issued on Jun. 13, 2017.
Application 14/877,925 is a continuation in part of application No. 14/225,356, filed on Mar. 25, 2014, abandoned.
Application 14/877,925 is a continuation in part of application No. 13/780,022, filed on Feb. 28, 2013, abandoned.
Application 14/877,925 is a continuation in part of application No. 13/683,604, filed on Nov. 21, 2012, abandoned.
Application 13/780,022 is a continuation of application No. 13/683,604, filed on Nov. 21, 2012, abandoned.
Application 13/683,604 is a continuation of application No. PCT/US2012/058578, filed on Oct. 3, 2012.
Application 13/780,022 is a continuation in part of application No. 13/335,043, filed on Dec. 22, 2011, granted, now 10,113,196, issued on Oct. 30, 2018.
Application 13/780,022 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/683,604 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/335,043 is a continuation in part of application No. 13/300,235, filed on Nov. 18, 2011, granted, now 10,017,812, issued on Jul. 10, 2018.
Application 13/300,235 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application 13/780,022 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Application 13/683,604 is a continuation in part of application No. 13/110,685, filed on May 18, 2011, granted, now 8,825,412, issued on Sep. 2, 2014.
Claims priority of provisional application 62/148,173, filed on Apr. 15, 2015.
Claims priority of provisional application 62/147,377, filed on Apr. 14, 2015.
Claims priority of provisional application 62/146,188, filed on Apr. 10, 2015.
Claims priority of provisional application 62/066,514, filed on Oct. 21, 2014.
Claims priority of provisional application 61/994,791, filed on May 16, 2014.
Claims priority of provisional application 61/987,407, filed on May 1, 2014.
Claims priority of provisional application 61/982,245, filed on Apr. 21, 2014.
Claims priority of provisional application 61/675,020, filed on Jul. 24, 2012.
Claims priority of provisional application 61/634,431, filed on Feb. 29, 2012.
Claims priority of provisional application 61/542,508, filed on Oct. 3, 2011.
Claims priority of provisional application 61/571,248, filed on Jun. 23, 2011.
Claims priority of provisional application 61/516,996, filed on Apr. 12, 2011.
Claims priority of provisional application 61/448,547, filed on Mar. 2, 2011.
Claims priority of provisional application 61/462,972, filed on Feb. 9, 2011.
Claims priority of provisional application 61/426,208, filed on Dec. 22, 2010.
Claims priority of provisional application 61/398,159, filed on Jun. 21, 2010.
Claims priority of provisional application 61/395,850, filed on May 18, 2010.
Prior Publication US 2022/0307086 A1, Sep. 29, 2022
1. A method for preparing a non-naturally occurring preparation of amplified DNA from a maternal blood sample or a fraction thereof useful for non-invasive prenatal aneuploidy determination, wherein the maternal blood sample is from a pregnant mother of a fetus, the method comprising:
(a) extracting cell-free DNA from the maternal blood sample or fraction thereof, wherein the extracted cell-free DNA comprises a mixture of fetal cell-free DNA and maternal cell-free DNA;
(b) preparing a non-naturally occurring preparation of amplified DNA by (i) hybridizing a plurality of circularizing probes to the extracted mixture of fetal and maternal cell-free DNA, wherein the circularizing probes bind to a plurality of target loci on one or more chromosomes of interest, and wherein the plurality of circularizing probes comprise at least 50 circularizing probes each targeting a different target loci, (ii) obtaining a plurality of circularized probes by ligation, wherein the circularized probes comprise sequences of the target loci, and (iii) amplifying the circularized probes by rolling-circle amplification; and
(c) analyzing the non-naturally occurring preparation of amplified DNA to measure the amounts of the target loci on the one or more chromosomes of interest from the fetal and maternal cell-free DNA.