US 12,221,650 B2
Method of detecting RNA
Seok Keun Cho, Incheon (KR)
Assigned to XENOHELIX CO., LTD, Jeonju-si (KR)
Filed by XENOHELIX CO., LTD, Jeonju-si (KR)
Filed on Dec. 29, 2020, as Appl. No. 17/136,793.
Claims priority of application No. 10-2020-0066932 (KR), filed on Jun. 3, 2020.
Prior Publication US 2021/0381034 A1, Dec. 9, 2021
Int. Cl. C12Q 1/68 (2018.01); C12Q 1/6825 (2018.01); C12Q 1/6855 (2018.01)
CPC C12Q 1/6825 (2013.01) [C12Q 1/6855 (2013.01); C12Q 2563/179 (2013.01); C12Q 2563/185 (2013.01)] 6 Claims
OG exemplary drawing
 
1. A method of detecting RNA, comprising:
a) hybridizing a sensor DNA comprising a complementary sequence of a target RNA to be detected with the target RNA such that the sensor DNA complementarily binds to the target RNA;
b) one cycle of polymerization with a polymerase using the target RNA as a template and the sensor DNA as a primer;
c) generating a plurality of amplicons by amplifying using a primer complementary to the strand polymerized in step b); and
d) analyzing the sequence of the plurality of amplicons,
d′) ligating the plurality of amplicons; and
e) analyzing a sequence of the ligated amplicons after step d′), and
wherein the respective amplicon that is amplified comprises a sequence complementary to a barcode region of the target RNA,
wherein the step b) is performed only once in the method of detecting RNA,
wherein the sensor DNA is in an amount of 500 amol to 1 fmol, and
wherein the primer in step c) has a phosphate group covalently attached to the 5′ end.