| CPC C12Q 1/18 (2013.01) [C12Q 1/04 (2013.01); G01N 2800/44 (2013.01)] | 10 Claims |
|
1. A method of determining antibiotic breakpoints for rapidly determining bacterial susceptibility of a species of bacteria to a cell wall synthesis inhibiting antibiotic comprising:
obtaining strains of the species of bacteria, the strains comprising one or more known classifications of susceptibility to the cell wall synthesis inhibiting antibiotic under a first methodology, wherein the first methodology comprises minimum inhibitory concentration standards determined through either diffusion, micro dilution testing, or an E-Test, wherein the one or more known classifications of susceptibility are selected from the group consisting of: resistant, intermediate, and susceptible;
incubating the one or more strains having known classification of susceptibility to the cell wall synthesis inhibiting antibiotic under a first methodology with multiple concentrations of the cell wall synthesis inhibiting antibiotic;
detecting the lowest concentration of cell wall synthesis inhibiting antibiotic that results in cell enlargement or an increase in cell size for each classification of susceptibility to the minimum inhibitory concentrations for the classification under the first methodology; and
setting modified antibiotic breakpoint concentrations at the detected values of the lowest concentration of cell wall synthesis inhibiting antibiotic that results in cell enlargement or an increase in cell size for each classification of susceptibility to the minimum inhibitory concentrations for the classification under the first methodology.
|