	US 12,221,645 B2
	Processes for producing reduced coenzyme Q₁₀
Jun Zhu, Nantong (CN); Bingrong Wang, Xiamen (CN); Fei Xu, Nantong (CN); Fei Xing, Nantong (CN); and Dan Li, Xiamen (CN)
Assigned to KINGDOMWAY BIOTECHNOLOGY (JIANGSU) CO., LTD., Nantong (CN); INNER MONGOLIA KINGDOMWAY PHARMACEUTICAL CO., LTD., Huhhot (CN); JIANGSU CHENGXIN PHARMACEUTICAL CO., LTD., Nantong (CN); and XIAMEN KINGDOMWAY GROUP COMPANY, Xiamen (CN)
Filed by KINGDOMWAY BIOTECHNOLOGY (JIANGSU) CO., LTD., Jiangsu (CN); INNER MONGOLIA KINGDOMWAY PHARMACEUTICAL CO., LTD., Inner Mongolia (CN); JIANGSU CHENGXIN PHARMACEUTICAL CO., LTD., Jiangsu (CN); and XIAMEN KINGDOMWAY GROUP COMPANY, Fujian (CN)
Filed on Jun. 28, 2023, as Appl. No. 18/343,727.
Application 18/343,727 is a continuation of application No. PCT/CN2023/096228, filed on May 25, 2023.
Prior Publication US 2024/0002891 A1, Jan. 4, 2024
Int. Cl. C12P 7/66 (2006.01); C12N 9/02 (2006.01)

CPC C12P 7/66 (2013.01) [C12N 9/0051 (2013.01); C12Y 108/01004 (2013.01); C12Y 108/01007 (2013.01); C12Y 108/01009 (2013.01)]

11 Claims

1. A process for producing reduced coenzyme Q₁₀(CoQ₁₀), comprising:

preparing a reaction mixture, which includes oxidized CoQ₁₀, a reductase, a supplement coenzyme, a coenzyme regeneration enzyme, and a substrate of the coenzyme regeneration enzyme, wherein the reductase comprises glutathione reductase (GR) derived from Ectothiorhodospira haloalkaliphil, and has the amino acid sequence of SEQ ID NO: 3, and the coenzyme regeneration enzyme comprises glucose dehydrogenase (GDH) derived from Bacillus subtilis, and has the amino acid sequence of SEQ ID NO: 4; and

providing a condition so that components of the reaction mixture react to produce the reduced CoQ₁₀.