US 12,221,644 B2
Production of non-native monounsaturated fatty acids in bacteria
Andreas W. Schirmer, South San Francisco, CA (US); Katherine Ann Murphy, South San Francisco, CA (US); and Erin Frances Perry, South San Francisco, CA (US)
Assigned to Genomatica, Inc., San Diego, CA (US)
Appl. No. 17/271,439
Filed by Genomatica, Inc., San Diego, CA (US)
PCT Filed Aug. 28, 2019, PCT No. PCT/US2019/048561
§ 371(c)(1), (2) Date Feb. 25, 2021,
PCT Pub. No. WO2020/047088, PCT Pub. Date Mar. 5, 2020.
Claims priority of provisional application 62/725,946, filed on Aug. 31, 2018.
Prior Publication US 2021/0189373 A1, Jun. 24, 2021
Int. Cl. C12N 9/02 (2006.01); C12N 9/04 (2006.01); C12N 9/10 (2006.01); C12N 9/16 (2006.01); C12N 9/88 (2006.01); C12P 7/6418 (2022.01); C12P 7/6481 (2022.01)
CPC C12P 7/6481 (2013.01) [C12N 9/0006 (2013.01); C12N 9/0008 (2013.01); C12N 9/001 (2013.01); C12N 9/1029 (2013.01); C12N 9/16 (2013.01); C12N 9/88 (2013.01); C12P 7/6418 (2013.01); C12Y 101/03013 (2013.01); C12Y 102/01003 (2013.01); C12Y 103/01038 (2013.01); C12Y 203/01041 (2013.01); C12Y 402/01059 (2013.01)] 20 Claims
 
1. A viable recombinant bacterium comprising a heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerase, wherein:
the viable recombinant bacterium produces a non-native monounsaturated fatty acid derivative when cultured;
the non-native monounsaturated fatty acid derivative has a double bond in a position characteristic of the heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerase;
the viable recombinant bacterium is a bacterium of taxonomic classification: Class gamma-proteobacteria;
the heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerase is derived from a bacterium of taxonomic classification: Class gamma-proteobacteria;
the heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerase is a Fab A homolog or a FabA-type dual 3-hydroxy-acyl-ACP dehydratase/isomerase; and
the heterologous dual 3-hydroxy-acyl-ACP dehydratase/isomerase replaces the native dual 3-hydroxy-acyl-ACP dehydratase/isomerase.