US 12,221,641 B2
Stevia rebaudiana kaurenoic acid hydroxylase variants for high efficiency production of rebaudiosides
Gale A. Wichmann, Emeryville, CA (US); Wenzong Li, Emeryville, CA (US); Sean Lund, Emeryville, CA (US); Shaina J. Jackson, Emeryville, CA (US); Chantal V. Garcia De Gonzalo, Emeryville, CA (US); Hailley Warbington, Emeryville, CA (US); Yi Xiong, Emeryville, CA (US); and Svetlana Alekseyevna Borisova, Emeryville, CA (US)
Assigned to AMYRIS, INC., Emeryville, CA (US)
Appl. No. 17/285,888
Filed by AMYRIS, INC., Emeryville, CA (US)
PCT Filed Oct. 14, 2019, PCT No. PCT/US2019/056153
§ 371(c)(1), (2) Date Apr. 15, 2021,
PCT Pub. No. WO2020/081468, PCT Pub. Date Apr. 23, 2020.
Claims priority of provisional application 62/745,900, filed on Oct. 15, 2018.
Prior Publication US 2021/0371892 A1, Dec. 2, 2021
Int. Cl. C12P 19/56 (2006.01); C12N 1/18 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/88 (2006.01); C12N 9/90 (2006.01); C12R 1/865 (2006.01)
CPC C12P 19/56 (2013.01) [C12N 1/185 (2021.05); C12N 9/0042 (2013.01); C12N 9/0073 (2013.01); C12N 9/1051 (2013.01); C12N 9/88 (2013.01); C12N 9/90 (2013.01); C07K 2319/03 (2013.01); C12R 2001/865 (2021.05); C12Y 106/02004 (2013.01); C12Y 114/13079 (2013.01); C12Y 114/14 (2013.01); C12Y 204/01017 (2013.01); C12Y 205/01001 (2013.01); C12Y 402/03019 (2013.01); C12Y 505/01012 (2013.01)] 3 Claims
OG exemplary drawing
 
1. A kaurenoic acid hydroxylase polypeptide with residue numbers according to SEQ ID NO: 1, comprising:
one, two, three, four, five, six, seven, eight, nine, or ten, of the following mutations: I166R, I153L, S158D, G306L, L232D, 1333V, 1350L, V316L, G447V, and M308L,
wherein the kaurenoic acid hydroxylase polypeptide is capable of increased conversion of kaurenoic acid to steviol compared to a kaurenoic acid hydroxylase polypeptide of SEQ ID NO:1.