	US 12,221,633 B2
	Modified reverse transcriptases
Arkadiusz Bibillo, Walnut Creek, CA (US); and Pranav Patel, Pleasanton, CA (US)
Assigned to BIO-RAD LABORATORIES, INC., Hercules, CA (US)
Filed by Bio-Rad Laboratories, Inc., Hercules, CA (US)
Filed on Jul. 7, 2023, as Appl. No. 18/348,544.
Application 18/348,544 is a continuation of application No. 17/055,801, granted, now 11,739,307, previously published as PCT/US2019/032701, filed on May 16, 2019.
Claims priority of provisional application 62/779,371, filed on Dec. 13, 2018.
Claims priority of provisional application 62/672,480, filed on May 16, 2018.
Prior Publication US 2024/0026316 A1, Jan. 25, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 9/12 (2006.01)

CPC C12N 9/1276 (2013.01) [C12Y 207/07049 (2013.01); C07K 2319/20 (2013.01)]

19 Claims

1. A non-naturally occurring enzyme, comprising (i) a finger domain derived from an R2 retrotransposon; (ii) a thumb domain derived from an R2 retrotransposon; and (iii) an endonuclease domain derived from an R2 retrotransposon, and an amino acid sequence with at least 95% identity to SEQ ID NO: 20.