	US 12,221,632 B2
	Virus lysis and nucleic acid preservation solution
Yuandong Liu, Guangzhou (CN); Wei Meng, Guangzhou (CN); Meiying Chen, Guangzhou (CN); Qin Huang, Guangzhou (CN); Qingqing Chen, Guangzhou (CN); and Ning Zhou, Guangzhou (CN)
Assigned to GUANGZHOU DONGSHENG BIOTECH CO., LTD., Guangzhou (CN)
Filed by GUANGZHOU DONGSHENG BIOTECH CO., LTD., Guangzhou (CN)
Filed on Jul. 30, 2021, as Appl. No. 17/389,371.
Application 17/389,371 is a continuation of application No. PCT/CN2021/074681, filed on Feb. 1, 2021.
Claims priority of application No. 202010571607.0 (CN), filed on Jun. 22, 2020.
Prior Publication US 2022/0017874 A1, Jan. 20, 2022
Int. Cl. C12N 7/00 (2006.01); C12N 15/10 (2006.01)

CPC C12N 7/00 (2013.01) [C12N 15/1013 (2013.01)]

13 Claims

1. A virus lysis and nucleic acid preservation solution, comprising the following components:

isopropanol or ethyl alcohol,

guanidine hydrochloride,

tris (hydroxymethyl) aminomethane hydrochloride,

ethylenediaminetetraacetic acid,

proteinase K and

magnetic beads, wherein a concentration of the guanidine hydrochloride is 5-6 mol/L, a concentration of the tris (hydroxymethyl) aminomethane hydrochloride is 10-100 mmol/L, a concentration of the ethylenediaminetetraacetic acid is 2-5 mmol/L, and a volume percentage of the isopropanol or ethyl alcohol is 5%-40%, wherein the magnetic beads are hydroxyl magnetic beads, and wherein the guanidine hydrochloride in the virus lysis and nucleic acid preservation solution does not cause proteinase K inactivation or aggregation of the magnetic beads.