	US 12,221,624 B2
	Systems and methods for producing retinal progenitors
Karl Tryggvason, Singapore (SG); Hwee Goon Tay, Singapore (SG); and Aida Moreno Moral, Singapore (SG)
Assigned to National University of Singapore, Singapore (SG)
Appl. No. 16/755,312
Filed by National University of Singapore, Singapore (SG)
PCT Filed Oct. 17, 2018, PCT No. PCT/SG2018/050517 § 371(c)(1), (2) Date Apr. 10, 2020, PCT Pub. No. WO2019/078783, PCT Pub. Date Apr. 25, 2019.
Application 16/755,312 is a continuation in part of application No. PCT/SG2017/050520, filed on Oct. 17, 2017.
Prior Publication US 2021/0189328 A1, Jun. 24, 2021
Int. Cl. C12N 5/0793 (2010.01); C12P 21/02 (2006.01)

CPC C12N 5/062 (2013.01) [C12P 21/02 (2013.01); C12N 2500/98 (2013.01); C12N 2501/13 (2013.01); C12N 2501/15 (2013.01); C12N 2501/415 (2013.01); C12N 2501/999 (2013.01); C12N 2506/45 (2013.01); C12N 2533/52 (2013.01)]

15 Claims

1. A method for producing a population of photoreceptor progenitors or photoreceptors, comprising:

plating pluripotent human stem cells on a cell culture surface having a laminin matrix thereon, wherein the laminin matrix comprises (i) a first laminin which is either laminin-323 or laminin-523, and (ii) a second laminin which is laminin-521, wherein the first laminin and the laminin-521 are each either an intact protein or a protein fragment; and

culturing the pluripotent human stem cells to obtain photoreceptor progenitors or photoreceptors that express RCVRN.