	US 12,221,619 B2
	Taurine supplemented cell culture medium and methods of use
Amy S. Johnson, Briarcliff Manor, NY (US); Meghan E. Casey, Ridgewood, NJ (US); Shadia Oshodi, Jersey City, NJ (US); and Shawn Lawrence, Nyack, NY (US)
Assigned to Regeneron Pharmaceuticals Inc., Tarrytown, NY (US)
Filed by Regeneron Pharmaceuticals, Inc., Tarrytown, NY (US)
Filed on Sep. 22, 2023, as Appl. No. 18/371,959.
Application 18/371,959 is a continuation of application No. 17/707,017, filed on Mar. 29, 2022.
Application 17/707,017 is a continuation of application No. 15/749,037, granted, now 11,312,936, issued on Apr. 26, 2022, previously published as PCT/US2016/045403, filed on Aug. 3, 2016.
Claims priority of provisional application 62/200,689, filed on Aug. 4, 2015.
Prior Publication US 2024/0018467 A1, Jan. 18, 2024
This patent is subject to a terminal disclaimer.
Int. Cl. C12N 5/00 (2006.01); C12N 15/79 (2006.01)

CPC C12N 5/0018 (2013.01) [C12N 15/79 (2013.01); C12N 2500/33 (2013.01); C12N 2500/74 (2013.01); C12N 2500/80 (2013.01)]

30 Claims

1. A method for culturing recombinant eukaryotic cells for improved production of a recombinant protein of interest, comprising the steps of:

(a) propagating cells in a defined cell culture medium during a growth phase, and

(b) supplementing the defined cell culture medium with about 0.09 mM to about 0.9 mM ornithine and about 0.1 mM to about 10 mM L-taurine, and expressing the recombinant protein of interest during production phase,

wherein the addition of L-taurine increases the titer of the recombinant protein of interest by at least 3% when compared to cells expressing the recombinant protein of interest in a cell culture medium containing less than 0.1 mM L-taurine and wherein said propagating cells during growth phase is performed at a temperature of about 35° C. to 38° C., and said expressing a recombinant protein of interest during the production phase is performed at a temperature of about 29° C. to 37° C., wherein the temperature of the growth phase is higher than the temperature of the production phase.