	US 12,221,614 B2
	Reprogramming vectors
Kinga Karbowniczek, Hampton (GB); Lisa Caproni, Hampton (GB); John Tite, Hampton (GB); Tristan McKay, Manchester (GB); and Christopher Thornton, Manchester (GB)
Assigned to Touchlight IP Limited, Hampton (GB)
Appl. No. 17/044,680
Filed by Touchlight IP Limited, Hampton (GB)
PCT Filed Apr. 5, 2019, PCT No. PCT/GB2019/051000 § 371(c)(1), (2) Date Oct. 1, 2020, PCT Pub. No. WO2019/193361, PCT Pub. Date Oct. 10, 2019.
Claims priority of application No. 1805683 (GB), filed on Apr. 5, 2018.
Prior Publication US 2021/0147869 A1, May 20, 2021
Int. Cl. C12N 15/85 (2006.01); C12N 5/074 (2010.01)

CPC C12N 15/85 (2013.01) [C12N 5/0696 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/606 (2013.01); C12N 2501/608 (2013.01); C12N 2506/1307 (2013.01)]

9 Claims

1. A method of producing induced pluripotent stem cells (iPSCs) comprising introducing one or more closed linear vector(s) encoding one or more reprogramming factors into a population of mature somatic cells, and culturing said mature somatic cells to effect expression of the one or more reprogramming factor(s), wherein said one or more closed linear vector(s) lacks sequences for chromosomal scaffold attachment.