US 12,221,613 B2
Approaches to dramatically increase rice productivity
Su-Ying Yeh, Chiayi (TW); Hsin-Hung Lin, Taipei (TW); Maurice S. B. Ku, Kaohsing (TW); and Wen-Hsiung Li, Yuanshan Township (TW)
Assigned to Academia Sinica, Taipei (TW)
Appl. No. 17/923,455
Filed by Academia Sinica, Taipei (TW)
PCT Filed Apr. 29, 2021, PCT No. PCT/US2021/029863
§ 371(c)(1), (2) Date Nov. 4, 2022,
PCT Pub. No. WO2021/225862, PCT Pub. Date Nov. 11, 2021.
Claims priority of provisional application 63/019,672, filed on May 4, 2020.
Prior Publication US 2023/0203521 A1, Jun. 29, 2023
Int. Cl. C12N 15/82 (2006.01)
CPC C12N 15/8269 (2013.01) [C12N 15/8265 (2013.01)] 15 Claims
 
1. A transgenic rice plant, comprising in its genome a recombinant DNA construct that contains a first nucleic acid having a sequence of a first Golden 2-like transcription factor (GLK) gene operably linked to its natural promoter and 5′ untranslated region (5′UTR), and a second nucleic acid having a sequence of a second GLK gene operably linked to its natural promoter and 5′UTR, wherein the first GLK gene is Zea mays GLK1, the second GLK gene is Zea mays GLK2, both the first GLK gene and the second GLK gene are expressed in the transgenic rice plant, and the transgenic rice plant exhibits at least a 50% increase in shoot biomass and grain yield, as compared to an untransformed wild-type rice plant.