	US 12,221,612 B2
	Plants having increased oil quality
Michael David Marks, Roseville, MN (US); Ratan Chopra, St. Paul, MN (US); Nicole Folstad, Minneapolis, MN (US); Donald L. Wyse, Wyoming, MN (US); and John C. Sedbrook, Bloomington, IL (US)
Assigned to Regents of the University of Minnesota, Minneapolis, MN (US); and The Board of Trustees of Illinois State University, Normal, IL (US)
Appl. No. 16/969,434
Filed by REGENTS OF THE UNIVERSITY OF MINNESOTA, Minneapolis, MN (US); and THE BOARD OF TRUSTEES OF ILLINOIS STATE UNIVERSITY, Normal, IL (US)
PCT Filed Feb. 12, 2019, PCT No. PCT/US2019/017660 § 371(c)(1), (2) Date Aug. 12, 2020, PCT Pub. No. WO2019/157504, PCT Pub. Date Aug. 15, 2019.
Claims priority of provisional application 62/629,387, filed on Feb. 12, 2018.
Prior Publication US 2020/0370062 A1, Nov. 26, 2020
Int. Cl. A01H 5/00 (2018.01); A01H 1/00 (2006.01); A01H 6/20 (2018.01); C12N 15/00 (2006.01); C12N 15/82 (2006.01)

CPC C12N 15/8247 (2013.01) [A01H 1/00 (2013.01); A01H 6/20 (2018.05); C12N 15/8213 (2013.01); C12Y 101/01098 (2013.01); C12Y 203/01199 (2015.07)]

14 Claims

1. A pennycress plant having reduced levels of polyunsaturated fatty acids (PUFAs) and/or increased levels of oleic acid, said pennycress plant comprising:

a genome comprising a modified reduced oleate desaturation 1 (ROD1) gene, wherein the modified ROD1 gene comprises a loss-of-function modification effective to knock-out expression levels of ROD1 polypeptides and/or ROD1 polypeptide activity;

wherein an unmodified coding sequence of the ROD1 gene encodes a ROD1 polypeptide having the amino acid sequence shown in SEQ ID NO:10; and

wherein the modification in the ROD1 gene is effective to reduce levels of PUFAs and/or increase levels of oleic acid in the plant compared to a corresponding pennycress plant lacking the modification in the ROD1 gene.