US 12,221,603 B2
System and method for production of on-demand proteins in a portable unit for point of care delivery
Govind Rao, Ellicott City, MD (US); Yordan Kostov, Columbia, MD (US); Leah Tolosa, Columbia, MD (US); Xudong Ge, Woodstock, MD (US); and Douglas Frey, Ellicott City, MD (US)
Assigned to University of Maryland, Baltimore County, Baltimore, MD (US)
Filed by University of Maryland, Baltimore County, Baltimore, MD (US)
Filed on Jun. 6, 2023, as Appl. No. 18/329,659.
Application 18/329,659 is a division of application No. 17/376,439, filed on Jul. 15, 2021, granted, now 11,702,625.
Application 17/376,439 is a division of application No. 16/506,079, filed on Jul. 9, 2019, abandoned.
Application 16/506,079 is a division of application No. 15/966,609, filed on Apr. 30, 2018, granted, now 10,435,664.
Application 15/966,609 is a continuation of application No. 15/095,305, filed on Apr. 11, 2016, granted, now 9,982,227.
Application 15/095,305 is a continuation in part of application No. 13/823,911, granted, now 9,388,373, previously published as PCT/US2012/028358, filed on Mar. 8, 2012.
Claims priority of provisional application 61/450,191, filed on Mar. 8, 2011.
Prior Publication US 2023/0323277 A1, Oct. 12, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 9/00 (2006.01); C12M 1/00 (2006.01); C12M 1/34 (2006.01)
CPC C12M 47/10 (2013.01) [C12M 41/12 (2013.01); C12M 47/12 (2013.01)] 18 Claims
 
1. A method of treating anemia in a patient in need thereof, said method comprising administering Erythropoetin (EPO) to the patient, wherein the EPO is synthesized on-demand in a bioprocessing system using cell-free protein expression, said bioprocessing system comprising:
a production module for producing non-purified EPO protein;
a purification module for receiving the non-purified EPO protein from the production module and for purifying the EPO protein; and
a processor for controlling and/or monitoring at least one of the production module and the purification module.