US 12,221,601 B2
Methods and systems for real-time assessment of cells in encapsulation devices pre-and post-transplantation
Klearchos K. Papas, Tucson, AZ (US)
Assigned to ARIZONA BOARD OF REGENTS ON BEHALF OF THE UNIVERSITY OF ARIZONA, Tucson, AZ (US)
Filed by Arizona Board of Regents on Behalf of the University of Arizona, Tucson, AZ (US)
Filed on Aug. 7, 2023, as Appl. No. 18/230,996.
Application 18/230,996 is a continuation of application No. 16/347,147, granted, now 11,746,318, previously published as PCT/US2017/060041, filed on Nov. 3, 2017.
Claims priority of provisional application 62/417,039, filed on Nov. 3, 2016.
Prior Publication US 2023/0383231 A1, Nov. 30, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C12M 1/34 (2006.01); C12M 1/00 (2006.01); C12M 1/02 (2006.01); C12M 1/12 (2006.01); C12N 5/071 (2010.01)
CPC C12M 41/34 (2013.01) [C12M 25/02 (2013.01); C12M 29/00 (2013.01); C12M 41/18 (2013.01); C12M 41/26 (2013.01); C12M 41/32 (2013.01); C12N 5/0676 (2013.01); C12N 2500/02 (2013.01)] 9 Claims
OG exemplary drawing
 
1. A system for measuring an oxygen consumption rate of cells, the system comprising:
a bioreactor comprising a first inlet and a first outlet, and a fluid media provided within the bioreactor;
a first oxygen sensor provided proximal to the first inlet and a second oxygen sensor provided proximal to the first outlet, wherein the first oxygen sensor and the second oxygen sensor are operable to detect oxygen levels of the fluid media and an oxygen consumption rate within the bioreactor based on a difference in oxygen measurements between the first oxygen sensor and the second oxygen sensor;
an implantable encapsulation device provided within and removable from the bioreactor, the encapsulation device being implantable within a patient, the encapsulation device comprising a channel, a lumen operable to receive cells, and a vascularization membrane at least partially encapsulating the lumen during and after implantation into the patient, wherein the viability of the cells is determinable during and after implantation into the patient, wherein the cells are operable to be at or above a pre-determined threshold for cell viability when the encapsulation device is implanted into the patient;
wherein the channel extends through the bioreactor and the encapsulation device, wherein the channel is configured to extend along and adjacent to the lumen, wherein the channel is partitioned from the fluid media of the bioreactor;
a gas exchanger provided between the first outlet and first inlet of the bioreactor operable to provide oxygen to the fluid media within the bioreactor; and
a pump for pumping and recirculating the fluid media from the first outlet of the bioreactor to the gas exchanger and the first inlet of the bioreactor and
a third sensor provided proximal to the first inlet and a fourth sensor provided proximal to the first outlet, wherein the third sensor and the fourth sensor are operable to measure glucose-stimulated insulin secretion within the bioreactor.