	US 12,221,597 B2
	Method of culturing cell, method of manufacturing cell support complex, cultured cell, and cell support complex
Fumihiko Kitagawa, Kanazawa (JP)
Assigned to NIKKISO CO., LTD., Tokyo (JP)
Filed by NIKKISO CO., LTD., Tokyo (JP)
Filed on Aug. 28, 2020, as Appl. No. 17/006,138.
Application 17/006,138 is a continuation of application No. PCT/JP2019/011389, filed on Mar. 19, 2019.
Claims priority of application No. 2018-054310 (JP), filed on Mar. 22, 2018.
Prior Publication US 2020/0392442 A1, Dec. 17, 2020
Int. Cl. C12M 1/00 (2006.01); C12M 1/06 (2006.01)

CPC C12M 23/20 (2013.01) [C12M 27/02 (2013.01)]

11 Claims

1. A method of culturing cells comprising:

culturing the cells in a cell suspension under repetitive cycles of stirring and rest while keeping the cells non-adherent to a culture vessel, wherein the culture vessel is treated by cell-repellent treatment or composed of a cell-repellent material,

forming at least one aggregate of the cells without applying a support in the cell suspension; and

subsequent to the steps of forming at least one aggregate and culturing the cells under repetitive cycles of stirring and rest, culturing the cells in a state of aggregate, with the cell suspension kept stirred.