US 12,221,487 B2
Nucleic acids encoding CX3CR1-binding polypeptides comprising immunoglobulin single variable domains
Sanjaya Singh, Sandy Hook, CT (US); Alisa K. Waterman, Branford, CT (US); Erik Depla, Destelbergen (BE); Toon Laeremans, Dworp-Beersel (BE); Diane Van Hoorick, Laarne (BE); and Cedric Jozef Néotère Ververken, Merelbeke (BE)
Assigned to Ablynx N.V., Ghent-Zwijnaarde (BE)
Filed by Ablynx N.V., Zwijnaarde (BE)
Filed on Jun. 9, 2022, as Appl. No. 17/836,473.
Application 15/242,639 is a division of application No. 14/682,131, filed on Apr. 9, 2015, granted, now 9,458,235, issued on Oct. 4, 2016.
Application 14/682,131 is a division of application No. 13/775,307, filed on Feb. 25, 2013, granted, now 9,035,029, issued on May 19, 2015.
Application 17/836,473 is a continuation of application No. 16/505,773, filed on Jul. 9, 2019, granted, now 11,384,151.
Application 16/505,773 is a continuation of application No. 15/720,792, filed on Sep. 29, 2017, granted, now 10,385,134, issued on Aug. 20, 2019.
Application 15/720,792 is a continuation of application No. 15/242,639, filed on Aug. 22, 2016, granted, now 9,783,612, issued on Oct. 10, 2017.
Claims priority of provisional application 61/603,622, filed on Feb. 27, 2012.
Prior Publication US 2023/0046772 A1, Feb. 16, 2023
Prior Publication US 2024/0026013 A2, Jan. 25, 2024
Prior Publication US 2024/0279349 A2, Aug. 22, 2024
Int. Cl. C07K 16/28 (2006.01); A61K 39/395 (2006.01); C07H 21/04 (2006.01); C12N 15/09 (2006.01); C12N 15/11 (2006.01); C12N 15/63 (2006.01); A61K 39/00 (2006.01); A61P 13/12 (2006.01); C07K 14/52 (2006.01); C07K 14/715 (2006.01)
CPC C07K 16/2866 (2013.01) [C07H 21/04 (2013.01); C07K 16/28 (2013.01); C12N 15/09 (2013.01); C12N 15/11 (2013.01); C12N 15/63 (2013.01); A61K 39/3955 (2013.01); A61K 2039/505 (2013.01); A61P 13/12 (2018.01); C07K 14/521 (2013.01); C07K 14/7158 (2013.01); C07K 2317/22 (2013.01); C07K 2317/24 (2013.01); C07K 2317/30 (2013.01); C07K 2317/35 (2013.01); C07K 2317/52 (2013.01); C07K 2317/565 (2013.01); C07K 2317/569 (2013.01); C07K 2317/76 (2013.01); C07K 2317/92 (2013.01); C07K 2317/94 (2013.01); C07K 2319/00 (2013.01); C07K 2319/30 (2013.01)] 20 Claims
 
1. A nucleic acid molecule encoding a polypeptide comprising an anti-CX3 chemokine receptor 1 (CX3CR1) immunoglobulin single variable domain and a half-life extending moiety, wherein said anti-CX3CR1 immunoglobulin single variable domain consists essentially of four framework regions (FR1, FR2, FR3 and FR4) and three complementary determining regions (CDR1, CDR2 and CDR3), wherein:
i) said CDR1 has the amino acid sequence of SEQ ID NO: 146;
ii) said CDR2 has an amino acid sequence that a) has at least 90% amino acid identity with the amino acid sequence of SEQ ID NO: 176 or b) has the amino acid sequence of SEQ ID NO: 176 or SEQ ID NO: 177; and
iii) said CDR3 has the amino acid sequence of SEQ ID NO: 191.