US 12,221,472 B2
Compositions and methods for the removal of biofilms
Steven D. Goodman, Hilliard, OH (US); and Lauren O. Bakaletz, Hilliard, OH (US)
Assigned to Research Institute at Nationwide Children's Hospital, Columbus, OH (US)
Filed by Research Institute at Nationwide Children's Hospital, Columbus, OH (US)
Filed on Feb. 14, 2023, as Appl. No. 18/168,824.
Application 18/168,824 is a division of application No. 17/552,986, filed on Dec. 16, 2021, granted, now 11,629,182.
Application 15/999,215 is a division of application No. 15/078,987, filed on Mar. 23, 2016, abandoned.
Application 17/552,986 is a continuation of application No. 15/999,215, filed on Aug. 16, 2018, granted, now 11,274,144, issued on Mar. 15, 2022.
Application 15/078,987 is a continuation in part of application No. 14/967,228, filed on Dec. 11, 2015, abandoned.
Application 14/967,228 is a continuation of application No. PCT/US2014/042201, filed on Jun. 12, 2014.
Claims priority of provisional application 62/199,952, filed on Jul. 31, 2015.
Claims priority of provisional application 61/834,846, filed on Jun. 13, 2013.
Prior Publication US 2023/0235030 A1, Jul. 27, 2023
This patent is subject to a terminal disclaimer.
Int. Cl. C07K 16/12 (2006.01); A01N 37/46 (2006.01); A61K 39/02 (2006.01); A61K 39/39 (2006.01); A61K 39/395 (2006.01); A61K 39/40 (2006.01); A61K 45/06 (2006.01); C07K 16/40 (2006.01); G01N 33/569 (2006.01); G01N 33/68 (2006.01); A61K 39/00 (2006.01)
CPC C07K 16/1242 (2013.01) [A01N 37/46 (2013.01); A61K 39/0208 (2013.01); A61K 39/39541 (2013.01); A61K 39/40 (2013.01); A61K 45/06 (2013.01); C07K 16/12 (2013.01); C07K 16/1232 (2013.01); C07K 16/40 (2013.01); G01N 33/56911 (2013.01); G01N 33/6854 (2013.01); G01N 33/6893 (2013.01); A61K 2039/505 (2013.01); C07K 2317/34 (2013.01); C07K 2317/76 (2013.01); G01N 2500/04 (2013.01); G01N 2800/24 (2013.01)] 11 Claims
 
1. An isolated polypeptide consisting of (a) an amino acid sequence selected from MATITKLDIIEYLSDKYHLS (SEQ ID NO: 348), KYHLSKQDTKNVVENFLEEI (SEQ ID NO: 349), FLEEIRLSLESGQDVKLSGF (SEQ ID NO: 350), KLSGFGNFELRDKSSRPGRN (SEQ ID NO: 351), RPGRNPKTGDVVPVSARRVV (SEQ ID NO: 352), or ARRVVTFKPGQKLRARVEKTK (SEQ ID NO: 353), and (b) a heterologous amino acid sequence selected from:
(i) up to 20 random amino acids on the amine terminus of the amino acid sequence in (a); or
(ii) up to 15 random amino acids on the carboxy terminus of the amino acid sequence in (a).