	US 12,221,399 B2
	Exiguobacterium indicum and application thereof in synthesis of nano-selenium
Yanyun Zhu, Jiangsu (CN); Hongmei Jin, Jiangsu (CN); Gary Banuelos, Fresno, CA (US); Yiwei Dong, Jiangsu (CN); Ning Zhu, Jiangsu (CN); and Xiangyang Yu, Jiangsu (CN)
Assigned to JIANGSU ACADEMY OF AGRICULTURAL SCIENCES, Jiangsu (CN)
Appl. No. 18/559,060
Filed by JIANGSU ACADEMY OF AGRICULTURAL SCIENCES, Jiangsu (CN)
PCT Filed Jul. 20, 2022, PCT No. PCT/CN2022/106875 § 371(c)(1), (2) Date Nov. 6, 2023, PCT Pub. No. WO2023/020191, PCT Pub. Date Feb. 23, 2023.
Claims priority of application No. 202110941393.6 (CN), filed on Aug. 17, 2021.
Prior Publication US 2024/0228391 A1, Jul. 11, 2024
Int. Cl. C05D 9/00 (2006.01); A61K 33/04 (2006.01); B82Y 40/00 (2011.01); C12N 1/20 (2006.01); C12P 3/00 (2006.01); C12R 1/01 (2006.01)

CPC C05D 9/00 (2013.01) [A61K 33/04 (2013.01); B82Y 40/00 (2013.01); C12N 1/20 (2013.01); C12N 1/205 (2021.05); C12P 3/00 (2013.01); C12R 2001/01 (2021.05)]

4 Claims

1. A method for synthesizing nano-selenium, comprising:

1) inoculating Exiguobacterium indicum with preservation number GDMCC NO. 61594 into a lysogeny broth (LB) liquid medium, and performing activated culture until OD₆₀₀is approximately equal to 1.0 to obtain activated bacteria liquid;

2) inoculating the activated bacteria liquid obtained in step 1) into a fermentation tank containing a fermentation medium at an amount of inoculation by a volume ratio of 2.5±0.2%, and carrying out fermentation until the bacterial cells content is 0.4-0.6 mg/mL to obtain fermented bacteria liquid,

wherein the fermentation medium is prepared by: adding up to 1 L deionized water to 5 g of yeast extract, 10 g of tryptone, and 10 g of NaCl; sterilizing, and then adding 1 M selenite solution to make a final concentration of 5 mM selenite in the medium; and

3) centrifuging the fermented bacteria liquid obtained in step 2), and collecting a precipitate that is the nano-selenium.