| CPC B01L 7/52 (2013.01) [B01L 2300/1805 (2013.01); B01L 2300/1844 (2013.01); B01L 2300/1894 (2013.01); C12Q 1/686 (2013.01)] | 13 Claims |

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1. An apparatus for thermal cycling, the apparatus comprising:
a first thermal mass;
a second thermal mass;
a retaining member;
a sample module;
at least one actuator;
and a
controller, wherein:
the controller is configured to control the at least one actuator;
the actuator is configured to move the first thermal mass and the second thermal mass from a first position to a second position;
the first thermal mass and the second thermal mass are in contact with the sample module in the first position;
the first thermal mass and the second thermal mass are not in contact with the sample module in the second position;
the retaining member is configured to retain the sample module in a fixed position as the as the actuator moves the first thermal mass and the second thermal mass from the first position to the second position; and
the sample module comprises:
a core layer;
a first film bonded to the core layer;
a second film bonded to the core layer;
an inlet;
a channel in fluid communication with the inlet;
a plurality of sample chambers in fluid communication with the channel; and
a plurality of air spring chambers containing air.
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