US 12,220,705 B2
Localized diagnostic testing module
Jasmin B. Farshi, San Jose, CA (US); Pan Zhang, San Jose, CA (US); Jonathan Barry Hirst, Sunnyvale, CA (US); Babak Ziaie, West Lafayette, IN (US); Bela Incze, Morgan Hill, CA (US); and Babar M. Koraishy, Livermore, CA (US)
Assigned to Amazon Technologies, Inc., Seattle, WA (US)
Filed by Amazon Technologies, Inc., Seattle, WA (US)
Filed on Mar. 24, 2022, as Appl. No. 17/703,792.
Claims priority of provisional application 63/254,656, filed on Oct. 12, 2021.
Prior Publication US 2023/0113495 A1, Apr. 13, 2023
Int. Cl. C12Q 1/686 (2018.01); B01L 1/00 (2006.01); B01L 3/00 (2006.01); B01L 7/00 (2006.01); G01N 33/487 (2006.01); G16H 10/40 (2018.01); G16H 40/40 (2018.01)
CPC B01L 3/5029 (2013.01) [B01L 1/50 (2013.01); B01L 3/5025 (2013.01); B01L 7/5255 (2013.01); G01N 33/48771 (2013.01); G01N 33/48792 (2013.01); G16H 10/40 (2018.01); G16H 40/40 (2018.01); B01L 2200/04 (2013.01); B01L 2200/0689 (2013.01); B01L 2200/082 (2013.01); B01L 2200/10 (2013.01); B01L 2200/16 (2013.01); B01L 2300/021 (2013.01); B01L 2300/027 (2013.01); B01L 2300/044 (2013.01); B01L 2300/0609 (2013.01); B01L 2300/0654 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0832 (2013.01); B01L 2300/1805 (2013.01); B01L 2300/1894 (2013.01); B01L 2400/0478 (2013.01); C12Q 1/686 (2013.01)] 20 Claims
 
1. A PCR testing module, comprising:
a main cabinet comprising an enclosure that houses a PCR machine;
a patient interface disposed on an exterior of the main cabinet, the patient interface configured to accept input from a user;
a sample input station accessible at the exterior of the main cabinet, the sample input station being configured to receive a sample collection device (SCD) comprising a saliva sample;
a barcode scanner configured to read a barcode on the SCD;
an oven within the enclosure configured to thermally lyse the saliva sample in the SCD;
an SCD processing mechanism configured to transfer a lysed microportion of the saliva sample and water into a PCR sample tube that comprises a lyophilized master mix;
a sonicator configured to ultrasonically mix the lysed microportion of the saliva sample, the water, and the lyophilized master mix in the PCR sample tube attached to the SCD;
a robotic manipulator configured to (i) separate the PCR sample tube from the SCD and discard the SCD into a biohazard waste bin within the cabinet, (ii) seal the PCR sample tube to output a sealed PCR tube, and (iii) transfer the sealed PCR sample tube to the PCR machine; and
a controller configured to:
cause the PCR machine to conduct a PCR test on the contents of the PCR sample tube;
determine a result of the PCR test; and
provide results of the PCR test.