US 12,220,704 B2
Concentrating particles in a microfluidic device
Ravi Kapur, Sharon, MA (US); Kyle C. Smith, Cambridge, MA (US); and Mehmet Toner, Charlestown, MA (US)
Assigned to The General Hospital Corporation, Boston, MA (US)
Filed by The General Hospital Corporation, Boston, MA (US)
Filed on Mar. 5, 2024, as Appl. No. 18/595,927.
Application 16/211,362 is a division of application No. 14/931,421, filed on Nov. 3, 2015, granted, now 10,150,116, issued on Dec. 11, 2018.
Application 18/595,927 is a continuation of application No. 17/335,510, filed on Jun. 1, 2021, granted, now 11,944,972.
Application 17/335,510 is a continuation of application No. 16/211,362, filed on Dec. 6, 2018, granted, now 11,052,393, issued on Jul. 6, 2021.
Claims priority of provisional application 62/074,213, filed on Nov. 3, 2014.
Claims priority of provisional application 62/074,315, filed on Nov. 3, 2014.
Prior Publication US 2024/0198339 A1, Jun. 20, 2024
Int. Cl. B01L 3/00 (2006.01); A61K 35/28 (2015.01); G01N 1/40 (2006.01); G01N 15/00 (2024.01); G01N 15/01 (2024.01); G01N 15/02 (2024.01); G01N 15/06 (2024.01); G01N 15/14 (2024.01); G01N 15/149 (2024.01)
CPC B01L 3/502761 (2013.01) [A61K 35/28 (2013.01); B01L 3/502715 (2013.01); B01L 3/502746 (2013.01); B01L 3/502753 (2013.01); G01N 1/4077 (2013.01); G01N 15/0255 (2013.01); G01N 15/0618 (2013.01); G01N 15/1484 (2013.01); B01L 3/502776 (2013.01); B01L 2200/0631 (2013.01); B01L 2200/0647 (2013.01); B01L 2200/0652 (2013.01); B01L 2200/0668 (2013.01); B01L 2200/0684 (2013.01); B01L 2200/12 (2013.01); B01L 2300/0681 (2013.01); B01L 2300/0877 (2013.01); B01L 2300/185 (2013.01); B01L 2400/0409 (2013.01); B01L 2400/0415 (2013.01); B01L 2400/043 (2013.01); B01L 2400/0436 (2013.01); B01L 2400/0457 (2013.01); B01L 2400/0487 (2013.01); B01L 2400/082 (2013.01); G01N 2001/4088 (2013.01); G01N 2015/0053 (2013.01); G01N 15/01 (2024.01); G01N 2015/0288 (2013.01); G01N 2015/1486 (2013.01); G01N 15/149 (2024.01); G01N 2015/1493 (2013.01)] 25 Claims
OG exemplary drawing
 
1. A microfluidic device comprising:
a first module comprising a first microfluidic channel having a sample inlet at a first end of the first microfluidic channel, wherein the first microfluidic channel is configured to shift particles above a specific size to a first product outlet at a second end of the first microfluidic channel; and
a second module comprising:
an array of islands in a second microfluidic channel, a buffer inlet in fluid communication with a first end of the second microfluidic channel, and a product inlet in fluid communication with a first product outlet and a first end of the second microfluidic channel, wherein:
the array of islands is arranged in one or more rows that extend along a longitudinal direction in a corresponding microfluidic channel,
each island in a row is spaced apart from an adjacent island in the row to form a siphoning channel, and
the array of islands is configured and arranged to shift portions of fluid through the siphoning channel between adjacent islands within a row to a waste outlet, and to shift particles above a specific size into a buffer flowing in the second microfluidic channel and to a second product outlet;
wherein the first module and the second module are serially arranged such that a sample fluid having particles above the specific size flows from the first module to the second module.